αB-crystallin is expressed in non-lenticular tissues and accumulates in Alexander's disease brain

Toru Iwaki, Akiko Kume-Iwaki, Ronald K.H. Liem, James E. Goldman

研究成果: Contribution to journalArticle査読

481 被引用数 (Scopus)

抄録

Rosenthal fibers (RFs) are abnormal inclusions within astrocytes, characteristic of Alexander's disease. We have previously isolated a 22 kd protein component of RFs from Alexander's disease brain. By Western blotting, we detected its equivalent in several rat organs, with the highest level in heart, and in a human astrocytoma cell line (U-373MG). A cDNA library established from U-373MG was screened with an anti-RF protein antibody. A partial cDNA clone encoding the lens protein αB-crystallin was isolated. The anti-RF protein antibodies react with lens αB-crystallin. Furthermore, the distribution of αB-crystallin mRNA in rat organs is consistent with the Western blots. Therefore, αB-crystallin is not lens-specific and it can accumulate in large amounts in astrocytes in pathological conditions.

本文言語英語
ページ(範囲)71-78
ページ数8
ジャーナルCell
57
1
DOI
出版ステータス出版済み - 4 7 1989
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生化学、遺伝学、分子生物学(全般)

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