β-Cyanoalanine synthase is a mitochondrial cysteine synthase-like protein in spinach and Arabidopsis

Yves Hatzfeld, Akiko Maruyama, Ahlert Schmidt, Masaaki Noji, Kimiharu Ishizawa, Kazuki Saito

研究成果: Contribution to journalArticle査読

143 被引用数 (Scopus)

抄録

β-Cyano-alanine synthase (CAS; EC 4.4.1.9) plays an important role in cyanide metabolism in plants. Although the enzymatic activity of β-cyano-Ala synthase has been detected in a variety of plants, no cDNA or gene has been identified so far. We hypothesized that the mitochondrial cysteine synthase (CS; EC 4.2.99.8) isoform, Bsas3, could actually be identical to CAS in spinach (Spinacia oleracea) and Arabidopsis. An Arabidopsis expressed sequence tag database was searched for putative Bsas3 homologs and four new CS-like isoforms, ARAth;Bsas1;1, ARAth;Bsas3;1, ARAth;Bsas4;1, and ARAth;Bsas4;2, were identified in the process. ARAth;Bsas3;1 protein was homologous to the mitochondrial SPIol;Bsas3;1 isoform from spinach, whereas ARAth;Bsas4;1 and ARAth;Bsas4;2 proteins defined a new class within the CS-like proteins family. In contrast to spinach SPIol;Bsas1;1 and SPIol;Bsas2;1 recombinant proteins, spinach SPIol;Bsas3;1 and Arabidopsis ARAth; Bsas3;1 recombinant proteins exhibited preferred substrate specificities for the CAS reaction rather than for the CS reaction, which identified these Bsas3 isoforms as CAS. Immunoblot studies supported this conclusion. This is the first report of the identification of CAS synthase-encoding cDNAs in a living organism. A new nomenclature for CS-like proteins in plants is also proposed.

本文言語英語
ページ(範囲)1163-1171
ページ数9
ジャーナルPlant physiology
123
3
DOI
出版ステータス出版済み - 7 2000
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生理学
  • 遺伝学
  • 植物科学

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