By digestion with an enzyme preparation derived from a culture filtrate of Cellulomonas sp., an unsaturated disaccharide was produced accompanied by the release of mannose and β1 → 2mannobiose from the acidic oligomer mixture that was obtained from the acidic polysaccharide of Fusarium sp. M7-1 by acetolysis. The unsaturated disaccharide produced was isolated and identified as 4-deoxy-L-threo-hex-4-enopyranouronosyl α(1 → 2)-D-galactose. The same unsaturated sugar linked to the polysaccharide was also produced accompanied by the release of mannose and β → 2mannobiose from the acidic polysaccharide by the enzyme digestion.
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