1-methyl-4-phenylpyridinium (MPP+) inhibits mitochondrial oxygen consumption mediated by succinate as well as malate in rat pheochromocytoma PC12 cells

E. Hasegawa, H. Asagami, Dongchon Kang, S. Minakami, K. Takeshige

研究成果: ジャーナルへの寄稿記事

4 引用 (Scopus)

抄録

When rat pheochromocytoma PC12 cells are cultured with 1 mM 1-methyl-4-phenylpyridinium (MPP+), the number of viable cells decreases to one third in 4 days while the number increases ten-fold without MPP+. Oxygen consumption by mitochondria in the presence of malate is inhibited about 80% by the treatment of the cells with MPP+ for 4 days. Unexpectedly, succinate-dependent oxygen consumption is also inhibited to essentially the same extent as malate-dependent one. These results suggest that the impairment of the respiration mediated by succinate as well as malate is important as a mechanism of MPP+-induced cell death.

元の言語英語
ページ(範囲)409-413
ページ数5
ジャーナルBiochemistry and Molecular Biology International
35
発行部数2
出版物ステータス出版済み - 1995

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1-Methyl-4-phenylpyridinium
PC12 Cells
Succinic Acid
Pheochromocytoma
Oxygen Consumption
Rats
Oxygen
Mitochondria
Cell death
Respiration
Cell Death
Cell Count
Cells
malic acid

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Genetics
  • Molecular Biology

これを引用

1-methyl-4-phenylpyridinium (MPP+) inhibits mitochondrial oxygen consumption mediated by succinate as well as malate in rat pheochromocytoma PC12 cells. / Hasegawa, E.; Asagami, H.; Kang, Dongchon; Minakami, S.; Takeshige, K.

:: Biochemistry and Molecular Biology International, 巻 35, 番号 2, 1995, p. 409-413.

研究成果: ジャーナルへの寄稿記事

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abstract = "When rat pheochromocytoma PC12 cells are cultured with 1 mM 1-methyl-4-phenylpyridinium (MPP+), the number of viable cells decreases to one third in 4 days while the number increases ten-fold without MPP+. Oxygen consumption by mitochondria in the presence of malate is inhibited about 80{\%} by the treatment of the cells with MPP+ for 4 days. Unexpectedly, succinate-dependent oxygen consumption is also inhibited to essentially the same extent as malate-dependent one. These results suggest that the impairment of the respiration mediated by succinate as well as malate is important as a mechanism of MPP+-induced cell death.",
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T1 - 1-methyl-4-phenylpyridinium (MPP+) inhibits mitochondrial oxygen consumption mediated by succinate as well as malate in rat pheochromocytoma PC12 cells

AU - Hasegawa, E.

AU - Asagami, H.

AU - Kang, Dongchon

AU - Minakami, S.

AU - Takeshige, K.

PY - 1995

Y1 - 1995

N2 - When rat pheochromocytoma PC12 cells are cultured with 1 mM 1-methyl-4-phenylpyridinium (MPP+), the number of viable cells decreases to one third in 4 days while the number increases ten-fold without MPP+. Oxygen consumption by mitochondria in the presence of malate is inhibited about 80% by the treatment of the cells with MPP+ for 4 days. Unexpectedly, succinate-dependent oxygen consumption is also inhibited to essentially the same extent as malate-dependent one. These results suggest that the impairment of the respiration mediated by succinate as well as malate is important as a mechanism of MPP+-induced cell death.

AB - When rat pheochromocytoma PC12 cells are cultured with 1 mM 1-methyl-4-phenylpyridinium (MPP+), the number of viable cells decreases to one third in 4 days while the number increases ten-fold without MPP+. Oxygen consumption by mitochondria in the presence of malate is inhibited about 80% by the treatment of the cells with MPP+ for 4 days. Unexpectedly, succinate-dependent oxygen consumption is also inhibited to essentially the same extent as malate-dependent one. These results suggest that the impairment of the respiration mediated by succinate as well as malate is important as a mechanism of MPP+-induced cell death.

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