TY - JOUR
T1 - 2-Amino-6-vinyl purine as a tool for post synthetic conjugation of DNA with radio-, spin-, fluorescence labels and peptides.
AU - Ali, Md Monsur
AU - Nagatsugi, Fumi
AU - Yamamoto, Fumihiko
AU - Maeda, Minoru
AU - Sasaki, Shigeki
N1 - Copyright:
This record is sourced from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
PY - 2002
Y1 - 2002
N2 - We have previously demonstrated that 2-amino-6-vinylpurine is an efficient cross-linking agent with high selectivity towards cytidine. In this study, we applied this nucleobase analog to versatile conjugation of oligodeoxynucleotides with radio-, spin-, fluorescence- and peptide labels. The oligodeoxynucleotides incorporating 2-amino-6-vinylpurine nucleoside were reacted with amino- bearing nucleophies in an excess amount to give the corresponding conjugates in good yield.
AB - We have previously demonstrated that 2-amino-6-vinylpurine is an efficient cross-linking agent with high selectivity towards cytidine. In this study, we applied this nucleobase analog to versatile conjugation of oligodeoxynucleotides with radio-, spin-, fluorescence- and peptide labels. The oligodeoxynucleotides incorporating 2-amino-6-vinylpurine nucleoside were reacted with amino- bearing nucleophies in an excess amount to give the corresponding conjugates in good yield.
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M3 - Article
C2 - 12903149
AN - SCOPUS:0041317456
SP - 149
EP - 150
JO - Nucleic acids research. Supplement (2001)
JF - Nucleic acids research. Supplement (2001)
IS - 2
ER -