3D co-cultures of keratinocytes and melanocytes and cytoprotective effects on keratinocytes against reactive oxygen species by insect virus-derived protein microcrystals

Junji Shimabukuro, Ayako Yamaoka, Ken Ichi Murata, Eiji Kotani, Tomoko Hirano, Yumiko Nakajima, Goichi Matsumoto, Hajime Mori

研究成果: ジャーナルへの寄稿記事

2 引用 (Scopus)

抄録

Stable protein microcrystals called polyhedra are produced by certain insect viruses. Cytokines, such as fibroblast growth factors (FGFs), can be immobilized within polyhedra. Here, we investigated three-dimensional (3D) co-cultures of keratinocytes and melanocytes on collagen gel containing FGF-2 and FGF-7 polyhedra. Melanocytes were observed to reside at the base of the 3D cell culture and melanin was also typically observed in the lower layer. The 3D cell culture model with FGF-2 and FGF-7 polyhedra was a useful in vitro model of the epidermis due to effective melanogenesis, proliferation and differentiation of keratinocytes. FGF-7 polyhedra showed a potent cytoprotective effect when keratinocytes were treated with menadione, which is a generator of reactive oxygen species. The cytoprotective effect was activated by the inositol triphosphate kinase-Akt pathway leading to upregulation of the antioxidant enzymes superoxide dismutase and peroxiredoxin 6.

元の言語英語
ページ(範囲)64-69
ページ数6
ジャーナルMaterials Science and Engineering C
42
DOI
出版物ステータス出版済み - 9 1 2014

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Fibroblast Growth Factor 7
Microcrystals
insects
microcrystals
fibroblasts
viruses
Fibroblasts
Viruses
Reactive Oxygen Species
polyhedrons
Fibroblast Growth Factor 2
proteins
Proteins
Cell culture
Oxygen
Peroxiredoxin VI
oxygen
Vitamin K 3
Fibroblast Growth Factors
Melanins

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

これを引用

3D co-cultures of keratinocytes and melanocytes and cytoprotective effects on keratinocytes against reactive oxygen species by insect virus-derived protein microcrystals. / Shimabukuro, Junji; Yamaoka, Ayako; Murata, Ken Ichi; Kotani, Eiji; Hirano, Tomoko; Nakajima, Yumiko; Matsumoto, Goichi; Mori, Hajime.

:: Materials Science and Engineering C, 巻 42, 01.09.2014, p. 64-69.

研究成果: ジャーナルへの寄稿記事

Shimabukuro, Junji ; Yamaoka, Ayako ; Murata, Ken Ichi ; Kotani, Eiji ; Hirano, Tomoko ; Nakajima, Yumiko ; Matsumoto, Goichi ; Mori, Hajime. / 3D co-cultures of keratinocytes and melanocytes and cytoprotective effects on keratinocytes against reactive oxygen species by insect virus-derived protein microcrystals. :: Materials Science and Engineering C. 2014 ; 巻 42. pp. 64-69.
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abstract = "Stable protein microcrystals called polyhedra are produced by certain insect viruses. Cytokines, such as fibroblast growth factors (FGFs), can be immobilized within polyhedra. Here, we investigated three-dimensional (3D) co-cultures of keratinocytes and melanocytes on collagen gel containing FGF-2 and FGF-7 polyhedra. Melanocytes were observed to reside at the base of the 3D cell culture and melanin was also typically observed in the lower layer. The 3D cell culture model with FGF-2 and FGF-7 polyhedra was a useful in vitro model of the epidermis due to effective melanogenesis, proliferation and differentiation of keratinocytes. FGF-7 polyhedra showed a potent cytoprotective effect when keratinocytes were treated with menadione, which is a generator of reactive oxygen species. The cytoprotective effect was activated by the inositol triphosphate kinase-Akt pathway leading to upregulation of the antioxidant enzymes superoxide dismutase and peroxiredoxin 6.",
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AU - Kotani, Eiji

AU - Hirano, Tomoko

AU - Nakajima, Yumiko

AU - Matsumoto, Goichi

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