8-Mercapto-Cyclic GMP Mediates Hydrogen Sulfide-Induced Stomatal Closure in Arabidopsis

Kenji Honda, Naotake Yamada, Riichiro Yoshida, Hideshi Ihara, Tomohiro Sawa, Takaaki Akaike, Sumio Iwai

研究成果: Contribution to journalArticle査読

43 被引用数 (Scopus)

抄録

Plants are exposed to hydrogen sulfide (H2S) both exogenously, as it exists as a pollutant gas in the environment, and endogenously, as it is synthesized in cells. H2S has recently been found to function as a gaseous signaling molecule, but its signaling cascade remains unknown. Here, we examined H2S-mediated guard cell signaling in Arabidopsis. The H2S donor GYY4137 (morpholin-4-ium-4-methoxyphenyl [morpholino] phosphinodithioate) induced stomatal closure, which peaked after 150 min at 1 μM or after 90 min at 10 and 100 μM. After reaching maximal closure, stomatal apertures gradually increased in size in response to further exposure to GYY4137. GYY4137 induced nitric oxide (NO) generation in guard cells, and GYY4137-induced stomatal closure was reduced by an NO scavenger and inhibitors of NO-producing enzymes. Mass spectrometry analyses showed that GYY4137 induces the synthesis of 8-nitro-cGMP and 8-mercapto-cGMP and that this synthesis is mediated by NO. In addition, 8-mercapto-cGMP triggered stomatal closure. Moreover, inhibitor and genetic studies showed that calcium, cADP ribose and slow anion channel 1 act downstream of 8-mercapto-cGMP. This study therefore demonstrates that 8-mercapto-cGMP mediates the H2S signaling cascade in guard cells.

本文言語英語
ページ(範囲)1481-1489
ページ数9
ジャーナルPlant and Cell Physiology
56
8
DOI
出版ステータス出版済み - 8 1 2015

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science
  • Cell Biology

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