800 fold signal enhancements by transient isotachophoresis for immunoassay of HSA on standard cross channel microchips

M. R. Mohamadi, L. Mahmoudian, N. Kaji, M. Tokeshi, Y. Baba

研究成果: Chapter in Book/Report/Conference proceedingConference contribution

抄録

A fully automated preconcentration and stacking method based on transient isotachophoresis (ITP) on a standard cross channel microchip made of poly (methylmethacrylate) PMMA has been reported. Previously reported ITP has been either on a special chip design or required a hydrodynamic pressure for sample injection and a pause in the electrophoresis process which caused low reproducibility in analysis. In this work sample injection, preconcentration and separation are done continuously and are controlled solely by a sequential voltage switching program. The ITP followed by nondenaturing gel electrophoresis was applied for immunoassay of HSA.

本文言語英語
ホスト出版物のタイトルProceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
編集者Jean-Louis Viovy, Patrick Tabeling, Stephanie Descroix, Laurent Malaquin
出版社Chemical and Biological Microsystems Society
ページ1402-1404
ページ数3
ISBN(電子版)9780979806407
出版ステータス出版済み - 2007
イベント11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 - Paris, フランス
継続期間: 10 7 200710 11 2007

出版物シリーズ

名前Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007

会議

会議11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
Countryフランス
CityParis
Period10/7/0710/11/07

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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