A CD36-related transmembrane protein is coordinated with an intracellular lipid-binding protein in selective carotenoid transport for cocoon coloration

Takashi Sakudoh, Tetsuya Iizuka, Junko Narukawa, Hideki Sezutsu, Isao Kobayashi, Seigo Kuwazaki, Yutaka Banno, Akitoshi Kitamura, Hiromu Sugiyama, Naoko Takada, Hirofumi Fujimoto, Keiko Kadono-Okuda, Kazuei Mita, Toshiki Tamura, Kimiko Yamamoto, Kozo Tsuchida

研究成果: ジャーナルへの寄稿記事

53 引用 (Scopus)

抄録

The transport pathway of specific dietary carotenoids from the midgut lumen to the silk gland in the silkworm, Bombyx mori, is a model system for selective carotenoid transport because several genetic mutants with defects in parts of this pathway have been identified that manifest altered cocoon pigmentation. In the wild-type silkworm, which has both genes, Yellow blood (Y) and Yellow cocoon (C), lutein is transferred selectively from the hemolymph lipoprotein to the silk gland cells where it is accumulated into the cocoon. The Y gene encodes an intracellular carotenoid-binding protein (CBP) containing a lipid-binding domain known as the steroidogenic acute regulatory protein-related lipid transfer domain. Positional cloning and transgenic rescue experiments revealed that the C gene encodes Cameo2, a transmembrane protein gene belonging to the CD36 family genes, some of which, such as the mammalian SR-BI and the fruit fly ninaD, are reported as lipoprotein receptors or implicated in carotenoid transport for visual system. In C mutant larvae, Cameo2 expression was strongly repressed in the silk gland in a specific manner, resulting in colorless silk glands and white cocoons. The developmental profile of Cameo2 expression, CBP expression, and lutein pigmentation in the silk gland of the yellow cocoon strain were correlated. We hypothesize that selective delivery of lutein to specific tissue requires the combination of two components: 1) CBP as a carotenoid transporter in cytosol and 2) Cameo2 as a transmembrane receptor on the surface of the cells.

元の言語英語
ページ(範囲)7739-7751
ページ数13
ジャーナルJournal of Biological Chemistry
285
発行部数10
DOI
出版物ステータス出版済み - 3 5 2010

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Carotenoids
Silk
Carrier Proteins
Lipids
Lutein
Bombyx
Genes
Proteins
Pigmentation
Lipoprotein Receptors
Cloning
Cell Surface Receptors
Fruits
Diptera
Cytosol
Larva
Organism Cloning
Fruit
Blood
Tissue

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

A CD36-related transmembrane protein is coordinated with an intracellular lipid-binding protein in selective carotenoid transport for cocoon coloration. / Sakudoh, Takashi; Iizuka, Tetsuya; Narukawa, Junko; Sezutsu, Hideki; Kobayashi, Isao; Kuwazaki, Seigo; Banno, Yutaka; Kitamura, Akitoshi; Sugiyama, Hiromu; Takada, Naoko; Fujimoto, Hirofumi; Kadono-Okuda, Keiko; Mita, Kazuei; Tamura, Toshiki; Yamamoto, Kimiko; Tsuchida, Kozo.

:: Journal of Biological Chemistry, 巻 285, 番号 10, 05.03.2010, p. 7739-7751.

研究成果: ジャーナルへの寄稿記事

Sakudoh, T, Iizuka, T, Narukawa, J, Sezutsu, H, Kobayashi, I, Kuwazaki, S, Banno, Y, Kitamura, A, Sugiyama, H, Takada, N, Fujimoto, H, Kadono-Okuda, K, Mita, K, Tamura, T, Yamamoto, K & Tsuchida, K 2010, 'A CD36-related transmembrane protein is coordinated with an intracellular lipid-binding protein in selective carotenoid transport for cocoon coloration', Journal of Biological Chemistry, 巻. 285, 番号 10, pp. 7739-7751. https://doi.org/10.1074/jbc.M109.074435
Sakudoh, Takashi ; Iizuka, Tetsuya ; Narukawa, Junko ; Sezutsu, Hideki ; Kobayashi, Isao ; Kuwazaki, Seigo ; Banno, Yutaka ; Kitamura, Akitoshi ; Sugiyama, Hiromu ; Takada, Naoko ; Fujimoto, Hirofumi ; Kadono-Okuda, Keiko ; Mita, Kazuei ; Tamura, Toshiki ; Yamamoto, Kimiko ; Tsuchida, Kozo. / A CD36-related transmembrane protein is coordinated with an intracellular lipid-binding protein in selective carotenoid transport for cocoon coloration. :: Journal of Biological Chemistry. 2010 ; 巻 285, 番号 10. pp. 7739-7751.
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abstract = "The transport pathway of specific dietary carotenoids from the midgut lumen to the silk gland in the silkworm, Bombyx mori, is a model system for selective carotenoid transport because several genetic mutants with defects in parts of this pathway have been identified that manifest altered cocoon pigmentation. In the wild-type silkworm, which has both genes, Yellow blood (Y) and Yellow cocoon (C), lutein is transferred selectively from the hemolymph lipoprotein to the silk gland cells where it is accumulated into the cocoon. The Y gene encodes an intracellular carotenoid-binding protein (CBP) containing a lipid-binding domain known as the steroidogenic acute regulatory protein-related lipid transfer domain. Positional cloning and transgenic rescue experiments revealed that the C gene encodes Cameo2, a transmembrane protein gene belonging to the CD36 family genes, some of which, such as the mammalian SR-BI and the fruit fly ninaD, are reported as lipoprotein receptors or implicated in carotenoid transport for visual system. In C mutant larvae, Cameo2 expression was strongly repressed in the silk gland in a specific manner, resulting in colorless silk glands and white cocoons. The developmental profile of Cameo2 expression, CBP expression, and lutein pigmentation in the silk gland of the yellow cocoon strain were correlated. We hypothesize that selective delivery of lutein to specific tissue requires the combination of two components: 1) CBP as a carotenoid transporter in cytosol and 2) Cameo2 as a transmembrane receptor on the surface of the cells.",
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AU - Kobayashi, Isao

AU - Kuwazaki, Seigo

AU - Banno, Yutaka

AU - Kitamura, Akitoshi

AU - Sugiyama, Hiromu

AU - Takada, Naoko

AU - Fujimoto, Hirofumi

AU - Kadono-Okuda, Keiko

AU - Mita, Kazuei

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AU - Yamamoto, Kimiko

AU - Tsuchida, Kozo

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