A comparative analysis of COI, LSU and UPA marker data for the Hawaiian florideophyte Rhodophyta: Implications for DNA barcoding of red algae

Alison R. Sherwood, Thomas Sauvage, Akira Kurihara, Kimberly Y. Conklin, Gemot G. Presting

研究成果: Contribution to journalArticle査読

33 被引用数 (Scopus)

抄録

A total of 290 florideophyte samples from the Hawaiian Rhodophyta Biodiversity Survey, spanning 17 orders of red algae, were sequenced for the mitochondrial COI DNA barcode, partial nuclear LSU rRNA gene, and plastid UPA rRNA marker. COI was A-T rich (> 60% overall) in comparison to the two rRNA markers, and was also the least conserved, with the third codon position showing the most substitution. Saturation was reached at F84 distances of approximately 0.11 for COI and 0.30 for LSU, but not at all for UPA, and some differences were found when orders or groups of families were individually examined. Rates of sequencing success for the three markers ranged from 46.8% for COI to 64.7% for UPA and 79.6% for LSU, indicating substantial differences in ease of data acquisition. Concatenation of marker sequences starting with the least saturated marker (UPA), and adding in order of degree of saturation (UPA+LSU, followed by UPA+LSU+COI) resulted in a strong increase in bootstrap support with neighbor-joining analysis, indicating that some phylogenetic utility can be gained from barcode-like sequences that are obtained for biodiversity surveys.

本文言語英語
ページ(範囲)451-465
ページ数15
ジャーナルCryptogamie, Algologie
31
4
出版ステータス出版済み - 11 1 2010
外部発表はい

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science
  • Plant Science

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