A comparative study and phage typing of silage-making Lactobacillus bacteriophages

Katsumi Doi, Ye Zhang, Yousuke Nishizaki, Akiko Umeda, Sadahiro Ohmomo, Seiya Ogata

研究成果: ジャーナルへの寄稿記事

11 引用 (Scopus)

抄録

To investigate basic characteristics of 10 virulent phages active on silage-making lactobacilli, morphological properties, host ranges, protein composition and genome characterization were separated into five groups based on host ranges and basic properties. The seven phages of groups I, II and V were active on Lactobacillus plantarum and Lactobacillus pentosus. Phage φPY4 (group III) infected both L. casei and Lactobacillus rhamnosus. Phage φPY5 (group IV) specifically infected Lactobacillus casei. Morphologically, three phages of groups I belonged to the Myoviridae family, while seven other phages of groups II, III and V belonged to the Siphoviridae family. SDS-PAGE profiles, restriction analysis, G+C contents of DNA and Dot blot hybridization revealed a high degree of homology in each group. Clustering derived from host range analysis was closely related to results of DNA and protein analyses. These phages may be applicable to phage typing for silage-making lactobacilli.

元の言語英語
ページ(範囲)518-525
ページ数8
ジャーナルJournal of Bioscience and Bioengineering
95
発行部数5
DOI
出版物ステータス出版済み - 1 1 2003

Fingerprint

Bacteriophage Typing
Bacteriophages
Silage
Lactobacillus
Host Specificity
Myoviridae
Siphoviridae
Lactobacillus rhamnosus
DNA
Lactobacillus casei
Lactobacillus plantarum
Proteins
Base Composition
Cluster Analysis
Polyacrylamide Gel Electrophoresis
Genome
Genes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

これを引用

A comparative study and phage typing of silage-making Lactobacillus bacteriophages. / Doi, Katsumi; Zhang, Ye; Nishizaki, Yousuke; Umeda, Akiko; Ohmomo, Sadahiro; Ogata, Seiya.

:: Journal of Bioscience and Bioengineering, 巻 95, 番号 5, 01.01.2003, p. 518-525.

研究成果: ジャーナルへの寄稿記事

Doi, Katsumi ; Zhang, Ye ; Nishizaki, Yousuke ; Umeda, Akiko ; Ohmomo, Sadahiro ; Ogata, Seiya. / A comparative study and phage typing of silage-making Lactobacillus bacteriophages. :: Journal of Bioscience and Bioengineering. 2003 ; 巻 95, 番号 5. pp. 518-525.
@article{3906803323004586956ac36be706f14a,
title = "A comparative study and phage typing of silage-making Lactobacillus bacteriophages",
abstract = "To investigate basic characteristics of 10 virulent phages active on silage-making lactobacilli, morphological properties, host ranges, protein composition and genome characterization were separated into five groups based on host ranges and basic properties. The seven phages of groups I, II and V were active on Lactobacillus plantarum and Lactobacillus pentosus. Phage φPY4 (group III) infected both L. casei and Lactobacillus rhamnosus. Phage φPY5 (group IV) specifically infected Lactobacillus casei. Morphologically, three phages of groups I belonged to the Myoviridae family, while seven other phages of groups II, III and V belonged to the Siphoviridae family. SDS-PAGE profiles, restriction analysis, G+C contents of DNA and Dot blot hybridization revealed a high degree of homology in each group. Clustering derived from host range analysis was closely related to results of DNA and protein analyses. These phages may be applicable to phage typing for silage-making lactobacilli.",
author = "Katsumi Doi and Ye Zhang and Yousuke Nishizaki and Akiko Umeda and Sadahiro Ohmomo and Seiya Ogata",
year = "2003",
month = "1",
day = "1",
doi = "10.1263/jbb.95.518",
language = "English",
volume = "95",
pages = "518--525",
journal = "Journal of Bioscience and Bioengineering",
issn = "1389-1723",
publisher = "The Society for Biotechnology, Japan",
number = "5",

}

TY - JOUR

T1 - A comparative study and phage typing of silage-making Lactobacillus bacteriophages

AU - Doi, Katsumi

AU - Zhang, Ye

AU - Nishizaki, Yousuke

AU - Umeda, Akiko

AU - Ohmomo, Sadahiro

AU - Ogata, Seiya

PY - 2003/1/1

Y1 - 2003/1/1

N2 - To investigate basic characteristics of 10 virulent phages active on silage-making lactobacilli, morphological properties, host ranges, protein composition and genome characterization were separated into five groups based on host ranges and basic properties. The seven phages of groups I, II and V were active on Lactobacillus plantarum and Lactobacillus pentosus. Phage φPY4 (group III) infected both L. casei and Lactobacillus rhamnosus. Phage φPY5 (group IV) specifically infected Lactobacillus casei. Morphologically, three phages of groups I belonged to the Myoviridae family, while seven other phages of groups II, III and V belonged to the Siphoviridae family. SDS-PAGE profiles, restriction analysis, G+C contents of DNA and Dot blot hybridization revealed a high degree of homology in each group. Clustering derived from host range analysis was closely related to results of DNA and protein analyses. These phages may be applicable to phage typing for silage-making lactobacilli.

AB - To investigate basic characteristics of 10 virulent phages active on silage-making lactobacilli, morphological properties, host ranges, protein composition and genome characterization were separated into five groups based on host ranges and basic properties. The seven phages of groups I, II and V were active on Lactobacillus plantarum and Lactobacillus pentosus. Phage φPY4 (group III) infected both L. casei and Lactobacillus rhamnosus. Phage φPY5 (group IV) specifically infected Lactobacillus casei. Morphologically, three phages of groups I belonged to the Myoviridae family, while seven other phages of groups II, III and V belonged to the Siphoviridae family. SDS-PAGE profiles, restriction analysis, G+C contents of DNA and Dot blot hybridization revealed a high degree of homology in each group. Clustering derived from host range analysis was closely related to results of DNA and protein analyses. These phages may be applicable to phage typing for silage-making lactobacilli.

UR - http://www.scopus.com/inward/record.url?scp=0037832668&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037832668&partnerID=8YFLogxK

U2 - 10.1263/jbb.95.518

DO - 10.1263/jbb.95.518

M3 - Article

C2 - 16233449

AN - SCOPUS:0037832668

VL - 95

SP - 518

EP - 525

JO - Journal of Bioscience and Bioengineering

JF - Journal of Bioscience and Bioengineering

SN - 1389-1723

IS - 5

ER -