A complete view of the genetic diversity of the Escherichia coli O-antigen biosynthesis gene cluster

Atsushi Iguchi, Sunao Iyoda, Taisei Kikuchi, Yoshitoshi Ogura, Keisuke Katsura, Makoto Ohnishi, Tetsuya Hayashi, Nicholas R. Thomson

研究成果: Contribution to journalArticle査読

98 被引用数 (Scopus)

抄録

The O antigen constitutes the outermost part of the lipopolysaccharide layer in Gram-negative bacteria. The chemical composition and structure of the O antigen show high levels of variation even within a single species revealing itself as serological diversity. Here, we present a complete sequence set for the O-antigen biosynthesis gene clusters (O-AGCs) from all 184 recognized Escherichia coli O serogroups. By comparing these sequences, we identified 161 well-defined O-AGCs. Based on the wzx/wzy or wzm/wzt gene sequences, in addition to 145 singletons, 37 serogroups were placed into 16 groups. Furthermore, phylogenetic analysis of all the E. coli O-serogroup reference strains revealed that the nearly one-quarter of the 184 serogroups were found in the ST10 lineage, which may have a unique genetic background allowing a more successful exchange of O-AGCs. Our data provide a complete view of the genetic diversity of O-AGCs in E. coli showing a stronger association between host phylogenetic lineage and O-serogroup diversification than previously recognized. These data will be a valuable basis for developing a systematic molecular O-typing scheme that will allow traditional typing approaches to be linked to genomic exploration of E. coli diversity.

本文言語英語
ページ(範囲)101-107
ページ数7
ジャーナルDNA Research
22
1
DOI
出版ステータス出版済み - 2 1 2015
外部発表はい

All Science Journal Classification (ASJC) codes

  • 分子生物学
  • 遺伝学

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