TY - JOUR
T1 - A complete view of the genetic diversity of the Escherichia coli O-antigen biosynthesis gene cluster
AU - Iguchi, Atsushi
AU - Iyoda, Sunao
AU - Kikuchi, Taisei
AU - Ogura, Yoshitoshi
AU - Katsura, Keisuke
AU - Ohnishi, Makoto
AU - Hayashi, Tetsuya
AU - Thomson, Nicholas R.
N1 - Publisher Copyright:
© The Author 2014. Published by Oxford University Press on behalf of Kazusa DNA Research Institute.
PY - 2015/2/1
Y1 - 2015/2/1
N2 - The O antigen constitutes the outermost part of the lipopolysaccharide layer in Gram-negative bacteria. The chemical composition and structure of the O antigen show high levels of variation even within a single species revealing itself as serological diversity. Here, we present a complete sequence set for the O-antigen biosynthesis gene clusters (O-AGCs) from all 184 recognized Escherichia coli O serogroups. By comparing these sequences, we identified 161 well-defined O-AGCs. Based on the wzx/wzy or wzm/wzt gene sequences, in addition to 145 singletons, 37 serogroups were placed into 16 groups. Furthermore, phylogenetic analysis of all the E. coli O-serogroup reference strains revealed that the nearly one-quarter of the 184 serogroups were found in the ST10 lineage, which may have a unique genetic background allowing a more successful exchange of O-AGCs. Our data provide a complete view of the genetic diversity of O-AGCs in E. coli showing a stronger association between host phylogenetic lineage and O-serogroup diversification than previously recognized. These data will be a valuable basis for developing a systematic molecular O-typing scheme that will allow traditional typing approaches to be linked to genomic exploration of E. coli diversity.
AB - The O antigen constitutes the outermost part of the lipopolysaccharide layer in Gram-negative bacteria. The chemical composition and structure of the O antigen show high levels of variation even within a single species revealing itself as serological diversity. Here, we present a complete sequence set for the O-antigen biosynthesis gene clusters (O-AGCs) from all 184 recognized Escherichia coli O serogroups. By comparing these sequences, we identified 161 well-defined O-AGCs. Based on the wzx/wzy or wzm/wzt gene sequences, in addition to 145 singletons, 37 serogroups were placed into 16 groups. Furthermore, phylogenetic analysis of all the E. coli O-serogroup reference strains revealed that the nearly one-quarter of the 184 serogroups were found in the ST10 lineage, which may have a unique genetic background allowing a more successful exchange of O-AGCs. Our data provide a complete view of the genetic diversity of O-AGCs in E. coli showing a stronger association between host phylogenetic lineage and O-serogroup diversification than previously recognized. These data will be a valuable basis for developing a systematic molecular O-typing scheme that will allow traditional typing approaches to be linked to genomic exploration of E. coli diversity.
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U2 - 10.1093/dnares/dsu043
DO - 10.1093/dnares/dsu043
M3 - Article
C2 - 25428893
AN - SCOPUS:84927913976
VL - 22
SP - 101
EP - 107
JO - DNA Research
JF - DNA Research
SN - 1340-2838
IS - 1
ER -