TY - JOUR
T1 - A Conjugative Linear Plasmid in Streptomyces laurentii ATCC31255
AU - Kinoshita-Iramina, Chizuru
AU - Ahara, Maki Kit
AU - Doi, Katsumi
AU - Ogata, Seiya
N1 - Funding Information:
Acknowledgments. This work was supported in part by a Grant-in-Aid for Scientific Research (B) from the Ministry of Education, Science, Sports, and Culture of Japan and the Waksman Foundation of Japan Inc. We are grateful to Dr. H. Kinashi (Faculty of Engineering, Hiroshima University) and Dr. K. Nakayama (Faculty of Dentistry, Kyushu University) for helpful advice on isolation of a linear plasmid, and to M. Ohara for critical reading of the manuscript.
PY - 1997/1
Y1 - 1997/1
N2 - Plasmid pSLL of Streptomyces laurentii ATCC31255 (wild-type strain P0) is a 93-kilobase linear DNA plasmid that carries a protein bound to each 5′ end of the DNA. It was self-transmitted to the pSLL-cured strain by conjugation in solid culture. The pSLL-cured strain carried a circular plasmid, pSLS, and showed a marked decrease in spore formation and thiostrepton productivity, owing to the pSLS. However, by retransmission of pSLL, these things reverted to levels seen in strain PO. Thus, plasmid pSLL suppressed the injurious effects of pSLS on the host mycelia.
AB - Plasmid pSLL of Streptomyces laurentii ATCC31255 (wild-type strain P0) is a 93-kilobase linear DNA plasmid that carries a protein bound to each 5′ end of the DNA. It was self-transmitted to the pSLL-cured strain by conjugation in solid culture. The pSLL-cured strain carried a circular plasmid, pSLS, and showed a marked decrease in spore formation and thiostrepton productivity, owing to the pSLS. However, by retransmission of pSLL, these things reverted to levels seen in strain PO. Thus, plasmid pSLL suppressed the injurious effects of pSLS on the host mycelia.
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U2 - 10.1271/bbb.61.1469
DO - 10.1271/bbb.61.1469
M3 - Article
C2 - 9339547
AN - SCOPUS:0031217870
SN - 0916-8451
VL - 61
SP - 1469
EP - 1473
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 9
ER -