A conserved SUMOylation signaling for cell cycle control in a holocentric species Bombyx mori

Zhiqing Li, Hiroaki Mon, Jian Xu, Li Zhu, Jae Man Lee, Takahiro Kusakabe

研究成果: Contribution to journalArticle査読

8 被引用数 (Scopus)

抄録

SUMOylation is an essential post-translational modification that regulates a variety of cellular processes including cell cycle progression. Although the SUMOylation pathway has been identified and investigated in many eukaryotes, the mechanisms of SUMOylation in regulating the functions of various substrates are still poorly understood. Here, we utilized a model species, the silkworm Bombyx mori that possesses holocentric chromosomes, to exploit the role of the SUMOylation system in cell cycle regulation. We identified all the components that are involved in the SUMOylation pathway in the silkworm genome. Our data revealed a cell cycle-dependent transcription of the SUMOylation genes, localization of the SUMOylation proteins, and abundance of the SUMOylation substrates in cultured silkworm cells. Importantly, the proliferation of the silkworm cells was strikingly inhibited by interference with SUMOylation genes expression, possibly due to an arrest of the SUMOylation-deficient cells at the G2/M phase. Furthermore, disruption of the SUMOylation genes induced the defects of holocentric chromosome congression and segregation during mitosis, which was consistent with high expressions of the SUMOylation genes and high enrichments of global SUMOylation at this stage, suggesting that the SUMOylation system in silkworm is essential for cell cycle regulation, with one particular role in mitosis.

本文言語英語
ページ(範囲)71-79
ページ数9
ジャーナルInsect Biochemistry and Molecular Biology
51
1
DOI
出版ステータス出版済み - 8 2014

All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学
  • 昆虫科学

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