A convolutional neural network uses microscopic images to differentiate between mouse and human cell lines and their radioresistant clones

Masayasu Toratani, Masamitsu Konno, Ayumu Asai, Jun Koseki, Koichi Kawamoto, Keisuke Tamari, Zhihao Li, Daisuke Sakai, Toshihiro Kudo, Taroh Satoh, Katsutoshi Sato, Daisuke Motooka, Daisuke Okuzaki, Yuichiro Doki, Masaki Mori, Kazuhiko Ogawa, Hideshi Ishii

研究成果: Contribution to journalArticle

6 引用 (Scopus)

抜粋

Artificial intelligence (AI) trained with a convolutional neural network (CNN) is a recent technological advancement. Previously, several attempts have been made to train AI using medical images for clinical applications. However, whether AI can distinguish microscopic images of mammalian cells has remained debatable. This study assesses the accuracy of image recognition techniques using the CNN to identify microscopic images. We also attempted to distinguish between mouse and human cells and their radioresistant clones. We used phase-contrast microscopic images of radioresistant clones from two cell lines, mouse squamous cell carcinoma NR-S1, and human cervical carcinoma ME-180. We obtained 10,000 images of each of the parental NR-S1 and ME-180 controls as well as radioresistant clones. We trained the CNN called VGG16 using these images and obtained an accuracy of 96%. Features extracted by the trained CNN were plotted using t-distributed stochastic neighbor embedding, and images of each cell line were well clustered. Overall, these findings suggest the utility of image recognition using AI for predicting minute differences among phase-contrast microscopic images of cancer cells and their radioresistant clones. Significance: This study demonstrates rapid and accurate identification of radioresistant tumor cells in culture using artifical intelligence; this should have applications in future preclinical cancer research.

元の言語英語
ページ(範囲)6703-6707
ページ数5
ジャーナルCancer Research
78
発行部数23
DOI
出版物ステータス出版済み - 12 1 2018
外部発表Yes

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

フィンガープリント A convolutional neural network uses microscopic images to differentiate between mouse and human cell lines and their radioresistant clones' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用

    Toratani, M., Konno, M., Asai, A., Koseki, J., Kawamoto, K., Tamari, K., Li, Z., Sakai, D., Kudo, T., Satoh, T., Sato, K., Motooka, D., Okuzaki, D., Doki, Y., Mori, M., Ogawa, K., & Ishii, H. (2018). A convolutional neural network uses microscopic images to differentiate between mouse and human cell lines and their radioresistant clones. Cancer Research, 78(23), 6703-6707. https://doi.org/10.1158/0008-5472.CAN-18-0653