抄録
The time-specific requirement of Rho proteins for the S phase progression of vascular endothelial cells was determined by reversibly introducing inhibitor proteins with a cell-penetrating peptide. We found evidence of the reversibility of protein transduction. The removal of extracellular protein caused the transduced protein to decay in a manner sensitive to low temperatures. The time required for a 50% decay correlated with the protein size. The time-specific transduction of the inhibitor proteins uncovered a critical period requiring Rho proteins in the G1-S transition phase. Reversible protein transduction may thus be a powerful tool to investigate the time-specific role of signaling proteins.
本文言語 | 英語 |
---|---|
ページ(範囲) | 149-154 |
ページ数 | 6 |
ジャーナル | FEBS Letters |
巻 | 570 |
号 | 1-3 |
DOI | |
出版ステータス | 出版済み - 7月 16 2004 |
!!!All Science Journal Classification (ASJC) codes
- 生物理学
- 構造生物学
- 生化学
- 分子生物学
- 遺伝学
- 細胞生物学