A critical period requiring Rho proteins for cell cycle progression uncovered by reversible protein transduction in endothelial cells

Katsuya Hirano, Mayumi Hirano, Junji Nishimura, Hideo Kanaide

研究成果: ジャーナルへの寄稿 › 記事

12 引用 (Scopus)

抄録

The time-specific requirement of Rho proteins for the S phase progression of vascular endothelial cells was determined by reversibly introducing inhibitor proteins with a cell-penetrating peptide. We found evidence of the reversibility of protein transduction. The removal of extracellular protein caused the transduced protein to decay in a manner sensitive to low temperatures. The time required for a 50% decay correlated with the protein size. The time-specific transduction of the inhibitor proteins uncovered a critical period requiring Rho proteins in the G1-S transition phase. Reversible protein transduction may thus be a powerful tool to investigate the time-specific role of signaling proteins.

元の言語英語
ページ（範囲）149-154
ページ数6
ジャーナルFEBS Letters
巻570
発行部数1-3
DOI
出版物ステータス出版済み - 7 16 2004

Fingerprint

Cell Cycle Proteins
Endothelial cells
Endothelial Cells
Proteins
S Phase
Cell-Penetrating Peptides
Protein S
Temperature

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

これを引用

A critical period requiring Rho proteins for cell cycle progression uncovered by reversible protein transduction in endothelial cells. / Hirano, Katsuya; Hirano, Mayumi; Nishimura, Junji; Kanaide, Hideo.

：: FEBS Letters, 巻 570, 番号 1-3, 16.07.2004, p. 149-154.

研究成果: ジャーナルへの寄稿 › 記事

Hirano, K, Hirano, M, Nishimura, J & Kanaide, H 2004, 'A critical period requiring Rho proteins for cell cycle progression uncovered by reversible protein transduction in endothelial cells', FEBS Letters, 巻. 570, 番号 1-3, pp. 149-154. https://doi.org/10.1016/j.febslet.2004.05.084
Hirano K, Hirano M, Nishimura J, Kanaide H. A critical period requiring Rho proteins for cell cycle progression uncovered by reversible protein transduction in endothelial cells. FEBS Letters. 2004 7 16;570(1-3):149-154. https://doi.org/10.1016/j.febslet.2004.05.084
Hirano, Katsuya ; Hirano, Mayumi ; Nishimura, Junji ; Kanaide, Hideo. / A critical period requiring Rho proteins for cell cycle progression uncovered by reversible protein transduction in endothelial cells. ：: FEBS Letters. 2004 ; 巻 570, 番号 1-3. pp. 149-154.
@article{9541fd60b3e0494586ab85d699cd4273,
title = "A critical period requiring Rho proteins for cell cycle progression uncovered by reversible protein transduction in endothelial cells",
abstract = "The time-specific requirement of Rho proteins for the S phase progression of vascular endothelial cells was determined by reversibly introducing inhibitor proteins with a cell-penetrating peptide. We found evidence of the reversibility of protein transduction. The removal of extracellular protein caused the transduced protein to decay in a manner sensitive to low temperatures. The time required for a 50{\%} decay correlated with the protein size. The time-specific transduction of the inhibitor proteins uncovered a critical period requiring Rho proteins in the G1-S transition phase. Reversible protein transduction may thus be a powerful tool to investigate the time-specific role of signaling proteins.",
author = "Katsuya Hirano and Mayumi Hirano and Junji Nishimura and Hideo Kanaide",
year = "2004",
month = "7",
day = "16",
doi = "10.1016/j.febslet.2004.05.084",
language = "English",
volume = "570",
pages = "149--154",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "1-3",

}

TY - JOUR

T1 - A critical period requiring Rho proteins for cell cycle progression uncovered by reversible protein transduction in endothelial cells

AU - Hirano, Katsuya

AU - Hirano, Mayumi

AU - Nishimura, Junji

AU - Kanaide, Hideo

PY - 2004/7/16

Y1 - 2004/7/16

N2 - The time-specific requirement of Rho proteins for the S phase progression of vascular endothelial cells was determined by reversibly introducing inhibitor proteins with a cell-penetrating peptide. We found evidence of the reversibility of protein transduction. The removal of extracellular protein caused the transduced protein to decay in a manner sensitive to low temperatures. The time required for a 50% decay correlated with the protein size. The time-specific transduction of the inhibitor proteins uncovered a critical period requiring Rho proteins in the G1-S transition phase. Reversible protein transduction may thus be a powerful tool to investigate the time-specific role of signaling proteins.

AB - The time-specific requirement of Rho proteins for the S phase progression of vascular endothelial cells was determined by reversibly introducing inhibitor proteins with a cell-penetrating peptide. We found evidence of the reversibility of protein transduction. The removal of extracellular protein caused the transduced protein to decay in a manner sensitive to low temperatures. The time required for a 50% decay correlated with the protein size. The time-specific transduction of the inhibitor proteins uncovered a critical period requiring Rho proteins in the G1-S transition phase. Reversible protein transduction may thus be a powerful tool to investigate the time-specific role of signaling proteins.

UR - http://www.scopus.com/inward/record.url?scp=3142534324&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3142534324&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2004.05.084

DO - 10.1016/j.febslet.2004.05.084

M3 - Article

VL - 570

SP - 149

EP - 154

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 1-3

ER -

文献にアクセス