A cryptic fimbrial gene in Serratia marcescens

T. Moriya, Shun-Ichiro Kawabata, Y. Mizunoe, K. Amako

研究成果: ジャーナルへの寄稿記事

1 引用 (Scopus)

抄録

The gene coding for the mannose-sensitive hemagglutinating fimbriae in Serratia marcescens US5 was cloned into Escherichia coli K4 with a cosmid vector system. One of the transformants, US5-1, expressed two morphologically distinct fimbriae, one that was 5-nm wide and one that was 3-nm wide. The latter fimbria was morphologically and serologically indistinguishable from that of strain US5. Genetic analysis of transformant US5-1 showed that the gene responsible for the 5-nm-wide fimbriae was located more than 10 kilobases away from the gene responsible for the 3-nm-wide fimbriae. The molecular size of the subunits of these two fimbriae, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, were 19 kilodaltons for the 3-nm-wide fimbriae and 20 kilodaltons for the 5-nm-wide fimbriae. Serologically, the 5-nm-wide fimbriae did not cross-react with monoclonal or polyclonal antibodies raised against the mannose-sensitive hemagglutinating fimbriae of strain US5. Strain EL101, which expressed only the 5-nm-wide fimbriae, did not agglutinate chicken or human erythrocytes. These experimental results suggest that the gene for the 5-nm-wide fimbriae is cryptic in strain US5 and is expressed in E. coli K4 only after it is moved by transformation.

元の言語英語
ページ(範囲)6629-6636
ページ数8
ジャーナルJournal of bacteriology
171
発行部数12
DOI
出版物ステータス出版済み - 1 1 1989

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Serratia marcescens
Mannose
Genes
Escherichia coli
Cosmids
Sodium Dodecyl Sulfate
Polyacrylamide Gel Electrophoresis
Chickens
Erythrocytes
Antibodies

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

これを引用

A cryptic fimbrial gene in Serratia marcescens. / Moriya, T.; Kawabata, Shun-Ichiro; Mizunoe, Y.; Amako, K.

:: Journal of bacteriology, 巻 171, 番号 12, 01.01.1989, p. 6629-6636.

研究成果: ジャーナルへの寄稿記事

Moriya, T. ; Kawabata, Shun-Ichiro ; Mizunoe, Y. ; Amako, K. / A cryptic fimbrial gene in Serratia marcescens. :: Journal of bacteriology. 1989 ; 巻 171, 番号 12. pp. 6629-6636.
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abstract = "The gene coding for the mannose-sensitive hemagglutinating fimbriae in Serratia marcescens US5 was cloned into Escherichia coli K4 with a cosmid vector system. One of the transformants, US5-1, expressed two morphologically distinct fimbriae, one that was 5-nm wide and one that was 3-nm wide. The latter fimbria was morphologically and serologically indistinguishable from that of strain US5. Genetic analysis of transformant US5-1 showed that the gene responsible for the 5-nm-wide fimbriae was located more than 10 kilobases away from the gene responsible for the 3-nm-wide fimbriae. The molecular size of the subunits of these two fimbriae, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, were 19 kilodaltons for the 3-nm-wide fimbriae and 20 kilodaltons for the 5-nm-wide fimbriae. Serologically, the 5-nm-wide fimbriae did not cross-react with monoclonal or polyclonal antibodies raised against the mannose-sensitive hemagglutinating fimbriae of strain US5. Strain EL101, which expressed only the 5-nm-wide fimbriae, did not agglutinate chicken or human erythrocytes. These experimental results suggest that the gene for the 5-nm-wide fimbriae is cryptic in strain US5 and is expressed in E. coli K4 only after it is moved by transformation.",
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