A cysteine protease inhibitor stored in the large granules of horseshoe crab hemocytes: Purification, characterization, cDNA cloning and tissue localization

Kishan Lal Agarwala, Shun-Ichiro Kawabata, Michimasa Hirata, Maseru Miyagi, Susumu Tsunasawa, Sadaaki Iwanaga

研究成果: ジャーナルへの寄稿記事

35 引用 (Scopus)

抄録

A cysteine protease inhibitor with an apparent M(r) = 12,600, designated limulus (L)-cystatin, was isolated from hemocyte lysates of the Japanese horseshoe crab (Tachypleus tridentatus), using two steps of chromatography, including dextran sulfate-agarose, and carboxymethylated papain-agarose. L-cystatin inhibits amidolytic activity of papain by forming a noncovalent 1:1 complex with an equilibrium constant (K(i)) of 0.08 nM. It also inhibits cathepsin L (K(i) = 0.17 nM) and ficin (K(i) = 0.52 nM), but not argingipain (a bacterial cysteine protease) and calpains. A cDNA for L-cystatin was isolated and the open reading frame coded for a mature protein of 114 amino acids, of which 99 residues were confirmed by peptide sequencing, L-cystatin shows significant sequence identities to members of the family 2 cystatin, such as bovine colostrum cystatin (33%) and human cystatin S (31%). Northern blotting revealed expression of the mRNA in hemocytes and slightly in heart but expression was negligible in hepatopancreas, intestine, stomach, and muscle. Immunoblotting revealed the localization to be in the large granules of hemocytes. Furthermore, L-cystatin has an antimicrobial activity against Gram-negative bacteria, which is much stronger than that of chicken egg white cystatin. These data suggest that the large granule-derived L-cystatin serves synergistically to accomplish an effective defense against invading microbes, together with other defense molecules that are released in response to external stimuli.

元の言語英語
ページ(範囲)85-94
ページ数10
ジャーナルJournal of Biochemistry
119
発行部数1
出版物ステータス出版済み - 1 1996

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Cystatins
Cysteine Proteinase Inhibitors
Horseshoe Crabs
Hemocytes
Cloning
Purification
Organism Cloning
Complementary DNA
Tissue
Papain
Sepharose
Salivary Cystatins
Ficain
Cathepsin K
Hepatopancreas
Cathepsin L
Dextran Sulfate
Colostrum
Calpain
Cysteine Proteases

All Science Journal Classification (ASJC) codes

  • Biochemistry

これを引用

A cysteine protease inhibitor stored in the large granules of horseshoe crab hemocytes : Purification, characterization, cDNA cloning and tissue localization. / Agarwala, Kishan Lal; Kawabata, Shun-Ichiro; Hirata, Michimasa; Miyagi, Maseru; Tsunasawa, Susumu; Iwanaga, Sadaaki.

:: Journal of Biochemistry, 巻 119, 番号 1, 01.1996, p. 85-94.

研究成果: ジャーナルへの寄稿記事

Agarwala, Kishan Lal ; Kawabata, Shun-Ichiro ; Hirata, Michimasa ; Miyagi, Maseru ; Tsunasawa, Susumu ; Iwanaga, Sadaaki. / A cysteine protease inhibitor stored in the large granules of horseshoe crab hemocytes : Purification, characterization, cDNA cloning and tissue localization. :: Journal of Biochemistry. 1996 ; 巻 119, 番号 1. pp. 85-94.
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abstract = "A cysteine protease inhibitor with an apparent M(r) = 12,600, designated limulus (L)-cystatin, was isolated from hemocyte lysates of the Japanese horseshoe crab (Tachypleus tridentatus), using two steps of chromatography, including dextran sulfate-agarose, and carboxymethylated papain-agarose. L-cystatin inhibits amidolytic activity of papain by forming a noncovalent 1:1 complex with an equilibrium constant (K(i)) of 0.08 nM. It also inhibits cathepsin L (K(i) = 0.17 nM) and ficin (K(i) = 0.52 nM), but not argingipain (a bacterial cysteine protease) and calpains. A cDNA for L-cystatin was isolated and the open reading frame coded for a mature protein of 114 amino acids, of which 99 residues were confirmed by peptide sequencing, L-cystatin shows significant sequence identities to members of the family 2 cystatin, such as bovine colostrum cystatin (33{\%}) and human cystatin S (31{\%}). Northern blotting revealed expression of the mRNA in hemocytes and slightly in heart but expression was negligible in hepatopancreas, intestine, stomach, and muscle. Immunoblotting revealed the localization to be in the large granules of hemocytes. Furthermore, L-cystatin has an antimicrobial activity against Gram-negative bacteria, which is much stronger than that of chicken egg white cystatin. These data suggest that the large granule-derived L-cystatin serves synergistically to accomplish an effective defense against invading microbes, together with other defense molecules that are released in response to external stimuli.",
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