A decapeptide, growth stimulator identified from natural rubber serum powder for Bifidobacterium bifidum

Shin Ichi Etoh, Kayoko Asamura, Azumi Obu, Kenji Sonomoto, Ayaaki Ishizaki

研究成果: Contribution to journalArticle査読

抄録

Natural rubber serum powder, which is by-product obtained in the production of latex rubber, has a strong growth-stimulating activity for Bifidobacterium bifidum JCM 1254. The retained fraction obtained by ultrafiltration (molecular weight cutoff 1000) showed a growth-stimulating activity in a dose-dependent manner on B12 assay medium with ammonium sulfate. One of the growth stimulators was purified from the retained fraction by acetone precipitation, solid-phase extraction with a hydrophobic pretreatment column, and multi-stage reversed-phase HPLC. An increase of 53-fold in the specific activity, and a recovery of 1.3% were obtained. The amino acid composition and N-terminal sequence analyses of this growth stimulator provided the structure of Ala-Thr-Pro-Glu-Lys-Glu-Glu-Pro-Thr-Ala. The molecular mass was 1075 by MALDI-TOF MS analysis. These results showed that this growth stimulator was a decapeptide with the sequence shown above. This is the first report that clarified the structure of an active peptide for the growth of Bifidobacterium.

本文言語英語
ページ(範囲)171-181
ページ数11
ジャーナルJournal of the Faculty of Agriculture, Kyushu University
45
1
出版ステータス出版済み - 11 2000

All Science Journal Classification (ASJC) codes

  • 農業および生物科学(その他)

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