A DNA size probe based on energy migration in crosslinked chromatin

Maged F. Serag, Noritada Kaji, Yukihiro Okamoto, Manabu Tokeshi, Yoshinobu Baba

研究成果: Chapter in Book/Report/Conference proceedingConference contribution

抄録

DNA sizing has a central role in virtually every aspect of physical genomic analysis. Although capillary gel electrophoresis adequately separates DNA samples, accurate sizing of short sequences is problematic. Here we introduce a simple methodology that affords sizing of short DNA sequences at 2nt resolution via wrapping and linking histone subunits with fluorophore-tagged ssDNA to extract a wrapping and linking ratio that is unique to the reported DNA size.

本文言語英語
ホスト出版物のタイトル15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
ページ58-60
ページ数3
1
出版ステータス出版済み - 12 1 2011
外部発表はい
イベント15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, 米国
継続期間: 10 2 201110 6 2011

その他

その他15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
国/地域米国
CitySeattle, WA
Period10/2/1110/6/11

All Science Journal Classification (ASJC) codes

  • 制御およびシステム工学

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