A fluorescent-labeled oligopeptide for monitoring PKA-mediated phosphorylation

Y. Ohuchi, Yoshiki Katayama, M. Maeda

研究成果: ジャーナルへの寄稿記事

6 引用 (Scopus)

抄録

A fluorescent-labeled oligopeptide (DACM-CLRRASLK-fluorescein), containing a consensus amino acid sequence (RRXSL) of cyclic AMP (cAMP) dependent protein kinase A (PKA) substrate-proteins, was designed. The fluorescent peptide was a good substrate of PKA, and the phosphorylation of its serin residue caused an intensive change in fluorescent intensity. We expect that the peptide will be useful as a fluorescent indicator for monitoring PKA activity in living cells.

元の言語英語
ページ(範囲)1905-1907
ページ数3
ジャーナルAnalyst
125
発行部数11
DOI
出版物ステータス出版済み - 12 4 2000

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Oligopeptides
Phosphorylation
Cyclic AMP-Dependent Protein Kinases
Proteins
protein
Monitoring
monitoring
peptide
Peptides
substrate
Substrates
Fluorescein
Amino acids
Amino Acid Sequence
amino acid
Cells
Amino Acids

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

これを引用

A fluorescent-labeled oligopeptide for monitoring PKA-mediated phosphorylation. / Ohuchi, Y.; Katayama, Yoshiki; Maeda, M.

:: Analyst, 巻 125, 番号 11, 04.12.2000, p. 1905-1907.

研究成果: ジャーナルへの寄稿記事

Ohuchi, Y. ; Katayama, Yoshiki ; Maeda, M. / A fluorescent-labeled oligopeptide for monitoring PKA-mediated phosphorylation. :: Analyst. 2000 ; 巻 125, 番号 11. pp. 1905-1907.
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