TY - JOUR
T1 - A four-dimensional organoid system to visualize cancer cell vascular invasion
AU - Yanagisawa, Kiminori
AU - Konno, Masamitsu
AU - Liu, Hao
AU - Irie, Shinji
AU - Mizushima, Tsunekazu
AU - Mori, Masaki
AU - Doki, Yuichiro
AU - Eguchi, Hidetoshi
AU - Matsusaki, Michiya
AU - Ishii, Hideshi
N1 - Funding Information:
Funding: This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology (17H04282; 17K19698; 18K16356; 18K16355; 18KK0251; 19K22658; 19K07688); AMED, Japan (16cm0106414h0001; 17cm0106414h0002), and JST-PRESTO (15655131). Partial support was received from Takeda Science Foundation, Senri Life Science Foundation, Osaka Cancer Society, Princess Takamatsu Cancer Research Fund, Yasuda Medical, Pancreas Research Foundation, Nakatomi Foundation of Japan, and Suzuken Memorial Foundation.
PY - 2020/11
Y1 - 2020/11
N2 - Vascular invasion of cancer is a critical step in cancer progression, but no drug has been developed to inhibit vascular invasion. To achieve the eradication of cancer metastasis, elucidation of the mechanism for vascular invasion and the development of innovative treatment methods are required. Here, a simple and reproducible vascular invasion model is established using a vascular organoid culture in a fibrin gel with collagen microfibers. Using this model, it was possible to observe and evaluate the cell dynamics and histological positional relationship of invasive cancer cells in four dimensions. Cancer-derived exosomes promoted the vascular invasion of cancer cells and loosened tight junctions in the vascular endothelium. As a new evaluation method, research using this vascular invasion mimic model will be advanced, and applications to the evaluation of the vascular invasion suppression e_ect of a drug are expected.
AB - Vascular invasion of cancer is a critical step in cancer progression, but no drug has been developed to inhibit vascular invasion. To achieve the eradication of cancer metastasis, elucidation of the mechanism for vascular invasion and the development of innovative treatment methods are required. Here, a simple and reproducible vascular invasion model is established using a vascular organoid culture in a fibrin gel with collagen microfibers. Using this model, it was possible to observe and evaluate the cell dynamics and histological positional relationship of invasive cancer cells in four dimensions. Cancer-derived exosomes promoted the vascular invasion of cancer cells and loosened tight junctions in the vascular endothelium. As a new evaluation method, research using this vascular invasion mimic model will be advanced, and applications to the evaluation of the vascular invasion suppression e_ect of a drug are expected.
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U2 - 10.3390/biology9110361
DO - 10.3390/biology9110361
M3 - Article
AN - SCOPUS:85094901349
VL - 9
SP - 1
EP - 14
JO - Biology
JF - Biology
SN - 2079-7737
IS - 11
M1 - 361
ER -