A galactosamine-mediated drug delivery carrier for targeted liver cancer therapy

Zheyu Shen, Wei Wei, Hideyuki Tanaka, Kazuhiro Kohama, Guanghui Ma, Toshiaki Dobashi, Yasuyuki Maki, Honghui Wang, Jingxiu Bi, Sheng Dai

研究成果: Contribution to journalArticle査読

61 被引用数 (Scopus)

抄録

In order to minimize the side effect of cancer chemotherapy, a novel galactosamine-mediated drug delivery carrier, galactosamine-conjugated albumin nanoparticles (GAL-AN), was developed for targeted liver cancer therapy. The albumin nanoparticles (AN) and doxorubicin-loaded AN (DOX-AN) were prepared by the desolvation of albumin in the presence of glutaraldehyde crosslinker. Morphological study indicated the spherical structure of these synthesized particles with an average diameter of around 200 nm. The functional ligand of galactosamine (GAL) was introduced onto the surfaces of AN and DOX-AN via carbodiimide chemistry to obtain GAL-AN and GAL-DOX-AN. Cellular uptake and kinetic studies showed that GAL-AN is able to be selectively incorporated into the HepG2 cells rather than AoSMC cells due to the existence of asialoglycoprotein receptors on HepG2 cell surface. The cytotoxicity, measured by MTT test, indicated that AN and GAL-AN are non-toxic and GAL-DOX-AN is more effective in HepG2 cell killing than that of DOX-AN. As such, our results implied that GAL-AN and GAL-DOX-AN have specific interaction with HepG2 cells via the recognition of GAL and asialoglycoprotein receptor, which renders GAL-AN a promising anticancer drug delivery carrier for liver cancer therapy.

本文言語英語
ページ(範囲)410-419
ページ数10
ジャーナルPharmacological Research
64
4
DOI
出版ステータス出版済み - 10 2011
外部発表はい

All Science Journal Classification (ASJC) codes

  • Pharmacology

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