A gene cluster for 6-deoxy-L-talan synthesis in Actinobacillus actinomycetemcomitans

Yoshio Nakano, Yasuo Yoshida, Yoshihisa Yamashita, Toshihiko Koga

研究成果: ジャーナルへの寄稿記事

27 引用 (Scopus)

抄録

The serotype c antigen of Actinobacillus actinomycetemcomitans consists of 6-deoxy-L-talose. A gene cluster involved in the synthesis of serotype- specific polysaccharide antigen was cloned from the chromosomal DNA of A. actinomycetemcomitans NCTC 9710 (serotype c). This cluster consisted of 17 open reading frames. Escherichia coli produced the polysaccharide that reacts with the serotype c-specific antibody when transformed with a plasmid containing the cluster. Comparing the structure of the gene cluster with a similar duster from A. actinomycetemcomitans Y4 (serotype b), which produces a polysaccharide consisting of L-rhamnose and D-fucose, revealed that a 5.7 kb region containing seven genes in the cluster from strain Y4 was replaced by a 3.8 kb region containing three genes in strain NCTC 9710. The results suggest that these region, as well as dTDP-6-deoxy-L-talose-forming dTDP-4- keto-L-rhamnose reductase, is essential to the production of extracellular polysaccharide specific to serotype c.

元の言語英語
ページ(範囲)409-414
ページ数6
ジャーナルBiochimica et Biophysica Acta - Gene Structure and Expression
1442
発行部数2-3
DOI
出版物ステータス出版済み - 11 8 1998

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Aggregatibacter actinomycetemcomitans
Multigene Family
Polysaccharides
Genes
Antigens
Rhamnose
Fucose
Escherichia coli
Plasmids
Open Reading Frames
Serogroup
6-deoxytalan
Antibodies
DNA

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

これを引用

A gene cluster for 6-deoxy-L-talan synthesis in Actinobacillus actinomycetemcomitans. / Nakano, Yoshio; Yoshida, Yasuo; Yamashita, Yoshihisa; Koga, Toshihiko.

:: Biochimica et Biophysica Acta - Gene Structure and Expression, 巻 1442, 番号 2-3, 08.11.1998, p. 409-414.

研究成果: ジャーナルへの寄稿記事

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