A gene cluster for 6-deoxy-L-talan synthesis in Actinobacillus actinomycetemcomitans

Yoshio Nakano, Yasuo Yoshida, Yoshihisa Yamashita, Toshihiko Koga

研究成果: ジャーナルへの寄稿学術誌査読

28 被引用数 (Scopus)


The serotype c antigen of Actinobacillus actinomycetemcomitans consists of 6-deoxy-L-talose. A gene cluster involved in the synthesis of serotype- specific polysaccharide antigen was cloned from the chromosomal DNA of A. actinomycetemcomitans NCTC 9710 (serotype c). This cluster consisted of 17 open reading frames. Escherichia coli produced the polysaccharide that reacts with the serotype c-specific antibody when transformed with a plasmid containing the cluster. Comparing the structure of the gene cluster with a similar duster from A. actinomycetemcomitans Y4 (serotype b), which produces a polysaccharide consisting of L-rhamnose and D-fucose, revealed that a 5.7 kb region containing seven genes in the cluster from strain Y4 was replaced by a 3.8 kb region containing three genes in strain NCTC 9710. The results suggest that these region, as well as dTDP-6-deoxy-L-talose-forming dTDP-4- keto-L-rhamnose reductase, is essential to the production of extracellular polysaccharide specific to serotype c.

ジャーナルBiochimica et Biophysica Acta - Gene Structure and Expression
出版ステータス出版済み - 11月 8 1998

!!!All Science Journal Classification (ASJC) codes

  • 構造生物学
  • 生物理学
  • 生化学
  • 遺伝学


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