TY - JOUR
T1 - A gene cluster for the synthesis of serotype d-specific polysaccharide antigen in Actinobacillus actinomycetemcomitans
AU - Nakano, Yoshio
AU - Yoshida, Yasuo
AU - Suzuki, Nao
AU - Yamashita, Yoshihisa
AU - Koga, Toshihiko
N1 - Funding Information:
We thank S. Asikainen for providing A. actinomycetemcomitans serotype d-strains, 392, 1344, and 3381. This work was supported in part by a Grant-in-Aid for Encouragement of Young Scientists 11771157 (Y.N.) and Grants-in-Aid of Scientific Research (A) 10307054 (T.K.) and (B) 11470452 (T.K.) from the Ministry of Education, Science, Sports and Culture, Tokyo, Japan.
PY - 2000/9/7
Y1 - 2000/9/7
N2 - The serotype d antigen of Actinobacillus actinomycetemcomitans consists of d-glucose, d-mannose, and l-rhamnose in a molar ratio of 1:2:1. A gene cluster involved in the synthesis of serotype-specific polysaccharide antigen was cloned from the chromosomal DNA of A. actinomycetemcomitans IDH 781 (serotype d). This cluster consisted of 12 open reading frames. Insertional inactivation of six genes in this cluster resulted in loss of ability of A. actinomycetemcomitans IDH 781 cells to produce the polysaccharide. Comparing the structure of the gene cluster with similar clusters from other serotypes of A. actinomycetemcomitans, showed that eight genes are unique to serotype d; the other four genes are involved in the biosynthesis of dTDP-l-rhamnose. These results suggest that the synthesis and structure of serotype d-specific polysaccharide of A. actinomycetemcomitans is quite different from those of other serotype strains. (C) 2000 Elsevier Science B.V.
AB - The serotype d antigen of Actinobacillus actinomycetemcomitans consists of d-glucose, d-mannose, and l-rhamnose in a molar ratio of 1:2:1. A gene cluster involved in the synthesis of serotype-specific polysaccharide antigen was cloned from the chromosomal DNA of A. actinomycetemcomitans IDH 781 (serotype d). This cluster consisted of 12 open reading frames. Insertional inactivation of six genes in this cluster resulted in loss of ability of A. actinomycetemcomitans IDH 781 cells to produce the polysaccharide. Comparing the structure of the gene cluster with similar clusters from other serotypes of A. actinomycetemcomitans, showed that eight genes are unique to serotype d; the other four genes are involved in the biosynthesis of dTDP-l-rhamnose. These results suggest that the synthesis and structure of serotype d-specific polysaccharide of A. actinomycetemcomitans is quite different from those of other serotype strains. (C) 2000 Elsevier Science B.V.
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U2 - 10.1016/S0167-4781(00)00179-2
DO - 10.1016/S0167-4781(00)00179-2
M3 - Article
C2 - 10978535
AN - SCOPUS:0034618612
VL - 1493
SP - 259
EP - 263
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
SN - 0167-4781
IS - 1-2
ER -