A gene cluster for the synthesis of serotype d-specific polysaccharide antigen in Actinobacillus actinomycetemcomitans

Yoshio Nakano, Yasuo Yoshida, Nao Suzuki, Yoshihisa Yamashita, Toshihiko Koga

研究成果: Contribution to journalArticle査読

21 被引用数 (Scopus)

抄録

The serotype d antigen of Actinobacillus actinomycetemcomitans consists of d-glucose, d-mannose, and l-rhamnose in a molar ratio of 1:2:1. A gene cluster involved in the synthesis of serotype-specific polysaccharide antigen was cloned from the chromosomal DNA of A. actinomycetemcomitans IDH 781 (serotype d). This cluster consisted of 12 open reading frames. Insertional inactivation of six genes in this cluster resulted in loss of ability of A. actinomycetemcomitans IDH 781 cells to produce the polysaccharide. Comparing the structure of the gene cluster with similar clusters from other serotypes of A. actinomycetemcomitans, showed that eight genes are unique to serotype d; the other four genes are involved in the biosynthesis of dTDP-l-rhamnose. These results suggest that the synthesis and structure of serotype d-specific polysaccharide of A. actinomycetemcomitans is quite different from those of other serotype strains. (C) 2000 Elsevier Science B.V.

本文言語英語
ページ(範囲)259-263
ページ数5
ジャーナルBiochimica et Biophysica Acta - Gene Structure and Expression
1493
1-2
DOI
出版ステータス出版済み - 9 7 2000

All Science Journal Classification (ASJC) codes

  • 構造生物学
  • 生物理学
  • 生化学
  • 遺伝学

フィンガープリント

「A gene cluster for the synthesis of serotype d-specific polysaccharide antigen in Actinobacillus actinomycetemcomitans」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル