A gene-delivery system specific for hepatoma cells and an intracellular kinase signal based on human liver-specific bionanocapsules and signal-responsive artificial polymer

Jun Oishi, Joohee Jung, Akira Tsuchiya, Riki Toita, Jeong Hun Kang, Takeshi Mori, Takuro Niidome, Katsuyuki Tanizawa, Shun'ichi Kuroda, Yoshiki Katayama

研究成果: ジャーナルへの寄稿記事

6 引用 (Scopus)

抄録

Recently, our group has proposed a novel gene-regulation system responding to cAMP-dependent protein kinase (PKA) that has been applied to living cells. In this study, human liver-specific bionanocapsules (BNCs) are used as a gene-delivery system to increase transfection efficiency and to target specific cell types. BNCs can efficiently deliver a target gene to human hepatocytes and hepatoma cells in vitro or in vivo. The combination of a signal-responsive gene-delivery system with BNCs led to an increase in the transfection efficiency and selectivity for hepatoma cells. Expression from the delivered gene was identified from PKA-activated hepatoma cells (HepG2), but not from colon tumor cells (WiDr). These results show that the combination of a gene-regulation system responding to an intracellular signal with BNC can be used for the selective treatment of human hepatoma cells.

元の言語英語
ページ(範囲)174-178
ページ数5
ジャーナルInternational Journal of Pharmaceutics
396
発行部数1-2
DOI
出版物ステータス出版済み - 8 1 2010

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Gene Transfer Techniques
Hepatocellular Carcinoma
Polymers
Phosphotransferases
Liver
Transfection
Genes
Hep G2 Cells
Cyclic AMP-Dependent Protein Kinases
Protein Kinases
Hepatocytes
Colon
Gene Expression
Neoplasms

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

これを引用

A gene-delivery system specific for hepatoma cells and an intracellular kinase signal based on human liver-specific bionanocapsules and signal-responsive artificial polymer. / Oishi, Jun; Jung, Joohee; Tsuchiya, Akira; Toita, Riki; Kang, Jeong Hun; Mori, Takeshi; Niidome, Takuro; Tanizawa, Katsuyuki; Kuroda, Shun'ichi; Katayama, Yoshiki.

:: International Journal of Pharmaceutics, 巻 396, 番号 1-2, 01.08.2010, p. 174-178.

研究成果: ジャーナルへの寄稿記事

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abstract = "Recently, our group has proposed a novel gene-regulation system responding to cAMP-dependent protein kinase (PKA) that has been applied to living cells. In this study, human liver-specific bionanocapsules (BNCs) are used as a gene-delivery system to increase transfection efficiency and to target specific cell types. BNCs can efficiently deliver a target gene to human hepatocytes and hepatoma cells in vitro or in vivo. The combination of a signal-responsive gene-delivery system with BNCs led to an increase in the transfection efficiency and selectivity for hepatoma cells. Expression from the delivered gene was identified from PKA-activated hepatoma cells (HepG2), but not from colon tumor cells (WiDr). These results show that the combination of a gene-regulation system responding to an intracellular signal with BNC can be used for the selective treatment of human hepatoma cells.",
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AU - Jung, Joohee

AU - Tsuchiya, Akira

AU - Toita, Riki

AU - Kang, Jeong Hun

AU - Mori, Takeshi

AU - Niidome, Takuro

AU - Tanizawa, Katsuyuki

AU - Kuroda, Shun'ichi

AU - Katayama, Yoshiki

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