A high-affinity peptide substrate for G protein-coupled receptor kinase 2 (GRK2)

Daisuke Asai, Masaharu Murata, Riki Toita, Takahito Kawano, Hideki Nakashima, Jeong Hun Kang

研究成果: Contribution to journalArticle査読

1 被引用数 (Scopus)

抄録

We synthesized a previously identified β-tubulin-derived G protein-coupled receptor kinase 2 (GKR2) peptide (GR-11-1; DEMEFTEAESNMN) and its amino-terminal extension (GR-11-1-N; GEGMDEMEFTEAESNMN) and carboxyl-terminal extension (GR-11-1-C; DEMEFTEAESNMNDLVSEYQ) peptides with the aim of finding a high-affinity peptide substrate for GRK2. GR-11-1-C showed high affinity for GRK2, but very low affinity for GKR5. Its specificity and sensitivity for GKR2 were greater than those of GR-11-1 and GR-11-1-N. These findings should be useful in designing tools for probing GKR2-mediated intracellular signaling pathways, as well as GRK2-specific drugs.

本文言語英語
ジャーナルAmino Acids
DOI
出版ステータス受理済み/印刷中 - 2019

All Science Journal Classification (ASJC) codes

  • 生化学
  • 臨床生化学
  • 有機化学

フィンガープリント

「A high-affinity peptide substrate for G protein-coupled receptor kinase 2 (GRK2)」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル