A High-Throughput and Solvent-free Method for Measurement of Natural Polyisoprene Content in Leaves by Fourier Transform Near Infrared Spectroscopy

Shinya Takeno, Takeshi Bamba, Yoshihisa Nakazawa, Eiichiro Fukusaki, Atsushi Okazawa, Akio Kobayashi

研究成果: ジャーナルへの寄稿記事

21 引用 (Scopus)

抄録

Commercial development of natural polyisoprene from polyisoprene-producing plants requires detailed knowledge on how to select high-polyisoprene-content lines and establish agronomic cultivation methods for achieving maximum polyisoprene yield. This development can be facilitated by a high-throughput quantification method for natural polyisoprene. In this paper, we describe the Fourier transform near infrared spectroscopy (FT-NIR) technique coupled with a partial least squares (PLS) regression model to quantify natural polyisoprene in Eucommia ulmoides leaves. PLS regression models are discussed with respect to linearity, root-mean-square error of estimation (RMSEE), and root-mean-square error of prediction (RMSEP). The best PLS regression model was obtained with second derivative NIR spectra in the region between 4000-6000 cm-1 (R2Y, 0.95; RMSEE, 0.25; RMSEP, 0.37). This is the first report to employ FT-NIR analysis for high throughput and solvent-free quantification of natural polyisoprene in leaves.

元の言語英語
ページ(範囲)537-540
ページ数4
ジャーナルJournal of Bioscience and Bioengineering
106
発行部数6
DOI
出版物ステータス出版済み - 12 1 2008

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Polyisoprenes
Near infrared spectroscopy
Near-Infrared Spectroscopy
Fourier Transform Infrared Spectroscopy
Least-Squares Analysis
Fourier transforms
Throughput
Eucommiaceae
Mean square error
Derivatives

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

これを引用

A High-Throughput and Solvent-free Method for Measurement of Natural Polyisoprene Content in Leaves by Fourier Transform Near Infrared Spectroscopy. / Takeno, Shinya; Bamba, Takeshi; Nakazawa, Yoshihisa; Fukusaki, Eiichiro; Okazawa, Atsushi; Kobayashi, Akio.

:: Journal of Bioscience and Bioengineering, 巻 106, 番号 6, 01.12.2008, p. 537-540.

研究成果: ジャーナルへの寄稿記事

Takeno, Shinya ; Bamba, Takeshi ; Nakazawa, Yoshihisa ; Fukusaki, Eiichiro ; Okazawa, Atsushi ; Kobayashi, Akio. / A High-Throughput and Solvent-free Method for Measurement of Natural Polyisoprene Content in Leaves by Fourier Transform Near Infrared Spectroscopy. :: Journal of Bioscience and Bioengineering. 2008 ; 巻 106, 番号 6. pp. 537-540.
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AU - Fukusaki, Eiichiro

AU - Okazawa, Atsushi

AU - Kobayashi, Akio

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