A highly efficient method for concentrating DNA from river water by combined coagulation and foam separation

Yoshihiro Suzuki, Yuki Imafuku, Masateru Nishiyama, Kotaro Teranishi, Atsushi Jikumaru, Kei Nukazawa, Yoshitoshi Ogura

研究成果: ジャーナルへの寄稿記事

抄録

Both Escherichia coli and Enterococci were collected in foam within 7 min from 500 mL of bacteria-spiked water by coagulation and foam separation using ferric chloride and milk casein. These bacterial DNA isolated in the 100 µL of extract from the foam more than 87.5% recovery using the DNeasy PowerWater® Kit. To test this method with water from three natural rivers, 0.67–2.70 µg of DNA were concentrated in 100 µL of extract from 1,000 mL of river water. When the DNA extract was subjected to 16S rRNA gene sequencing analysis, information on the bacterial flora could be obtained.

元の言語英語
ページ(範囲)3128-3134
ページ数7
ジャーナルSeparation Science and Technology (Philadelphia)
54
発行部数18
DOI
出版物ステータス受理済み/印刷中 - 1 1 2019

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Coagulation
Foams
DNA
Rivers
Water
Information analysis
Casein
Bacterial DNA
Caseins
Escherichia coli
Bacteria
Genes
Recovery

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)
  • Process Chemistry and Technology
  • Filtration and Separation

これを引用

A highly efficient method for concentrating DNA from river water by combined coagulation and foam separation. / Suzuki, Yoshihiro; Imafuku, Yuki; Nishiyama, Masateru; Teranishi, Kotaro; Jikumaru, Atsushi; Nukazawa, Kei; Ogura, Yoshitoshi.

:: Separation Science and Technology (Philadelphia), 巻 54, 番号 18, 12.12.2019, p. 3128-3134.

研究成果: ジャーナルへの寄稿記事

Suzuki, Yoshihiro ; Imafuku, Yuki ; Nishiyama, Masateru ; Teranishi, Kotaro ; Jikumaru, Atsushi ; Nukazawa, Kei ; Ogura, Yoshitoshi. / A highly efficient method for concentrating DNA from river water by combined coagulation and foam separation. :: Separation Science and Technology (Philadelphia). 2019 ; 巻 54, 番号 18. pp. 3128-3134.
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