A highly efficient method for concentrating DNA from river water by combined coagulation and foam separation

Yoshihiro Suzuki, Yuki Imafuku, Masateru Nishiyama, Kotaro Teranishi, Atsushi Jikumaru, Kei Nukazawa, Yoshitoshi Ogura

研究成果: Contribution to journalArticle

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Both Escherichia coli and Enterococci were collected in foam within 7 min from 500 mL of bacteria-spiked water by coagulation and foam separation using ferric chloride and milk casein. These bacterial DNA isolated in the 100 µL of extract from the foam more than 87.5% recovery using the DNeasy PowerWater® Kit. To test this method with water from three natural rivers, 0.67–2.70 µg of DNA were concentrated in 100 µL of extract from 1,000 mL of river water. When the DNA extract was subjected to 16S rRNA gene sequencing analysis, information on the bacterial flora could be obtained.

元の言語英語
ページ(範囲)3128-3134
ページ数7
ジャーナルSeparation Science and Technology (Philadelphia)
54
発行部数18
DOI
出版物ステータス出版済み - 12 12 2019

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)
  • Process Chemistry and Technology
  • Filtration and Separation

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