A highly toxic coplanar polychlorinated biphenyl compound suppresses Δ5 and Δ6 desaturase activities which play key roles in arachidonic acid synthesis in rat liver

Kimihiko Matsusue, Yuji Ishii, Noritaka Ariyoshi, Kazuta Oguri

研究成果: ジャーナルへの寄稿記事

26 引用 (Scopus)

抄録

The effect of 3,3',4,4',5-pentachlorobiphenyl (PenCB) on the synthesis of unsaturated fatty acids was studied in male Wistar rats. The arachidonic acid (20:4) content in the total lipids of liver homogenates was significantly reduced on day 5 of PenCB administration, while those of linoleic acid (18:2) and bishomo-γ-linolenic acid (20:3) were increased. These changes in the total lipids of liver homogenates were observed following doses of PenCB ranging from 0.5 to 25 mg/kg of body weight. The same changes in these fatty acids were seen with four subtypes of microsomal glycerophospholipids in the liver. The marked reduction in the molar ratio of 20:4 to 18:2 in the lipids suggests alteration of the activity of the enzymes responsible for the synthesis of unsaturated fatty acids. The activity of Δ5 and Δ6 desaturases (arachidonic acid synthetase) in the liver microsomes was 17 and 13% of that of pair-fed control animals, whereas the activity of 1- acylglycerophosphorylcholine or 1-acylglycerophosphate acyltransferase, which transfers 20:4 or 18:2 to phospholipids, was not affected by the treatment. Thus, the reduction in the level of 20:4 that was observed can be explained by a reduction in desaturase activity. These results are evidence that the coplanar PenCB has a significant effect on the reduced synthesis of physiologically essential long-chain unsaturated fatty acids.

元の言語英語
ページ(範囲)1158-1165
ページ数8
ジャーナルChemical Research in Toxicology
12
発行部数12
DOI
出版物ステータス出版済み - 1999

Fingerprint

Biphenyl Compounds
Poisons
Polychlorinated Biphenyls
Unsaturated Fatty Acids
Arachidonic Acid
Liver
Rats
3,4,5,3',4'-pentachlorobiphenyl
Lipids
1-Acylglycerol-3-Phosphate O-Acyltransferase
Glycerophospholipids
alpha-Linolenic Acid
Liver Microsomes
Linoleic Acid
Ligases
Wistar Rats
Phospholipids
Fatty Acids
Body Weight
Animals

All Science Journal Classification (ASJC) codes

  • Drug Discovery
  • Organic Chemistry
  • Chemistry(all)
  • Toxicology
  • Health, Toxicology and Mutagenesis

これを引用

A highly toxic coplanar polychlorinated biphenyl compound suppresses Δ5 and Δ6 desaturase activities which play key roles in arachidonic acid synthesis in rat liver. / Matsusue, Kimihiko; Ishii, Yuji; Ariyoshi, Noritaka; Oguri, Kazuta.

:: Chemical Research in Toxicology, 巻 12, 番号 12, 1999, p. 1158-1165.

研究成果: ジャーナルへの寄稿記事

@article{81334b4e39144fdea68cc744265084d6,
title = "A highly toxic coplanar polychlorinated biphenyl compound suppresses Δ5 and Δ6 desaturase activities which play key roles in arachidonic acid synthesis in rat liver",
abstract = "The effect of 3,3',4,4',5-pentachlorobiphenyl (PenCB) on the synthesis of unsaturated fatty acids was studied in male Wistar rats. The arachidonic acid (20:4) content in the total lipids of liver homogenates was significantly reduced on day 5 of PenCB administration, while those of linoleic acid (18:2) and bishomo-γ-linolenic acid (20:3) were increased. These changes in the total lipids of liver homogenates were observed following doses of PenCB ranging from 0.5 to 25 mg/kg of body weight. The same changes in these fatty acids were seen with four subtypes of microsomal glycerophospholipids in the liver. The marked reduction in the molar ratio of 20:4 to 18:2 in the lipids suggests alteration of the activity of the enzymes responsible for the synthesis of unsaturated fatty acids. The activity of Δ5 and Δ6 desaturases (arachidonic acid synthetase) in the liver microsomes was 17 and 13{\%} of that of pair-fed control animals, whereas the activity of 1- acylglycerophosphorylcholine or 1-acylglycerophosphate acyltransferase, which transfers 20:4 or 18:2 to phospholipids, was not affected by the treatment. Thus, the reduction in the level of 20:4 that was observed can be explained by a reduction in desaturase activity. These results are evidence that the coplanar PenCB has a significant effect on the reduced synthesis of physiologically essential long-chain unsaturated fatty acids.",
author = "Kimihiko Matsusue and Yuji Ishii and Noritaka Ariyoshi and Kazuta Oguri",
year = "1999",
doi = "10.1021/tx990104r",
language = "English",
volume = "12",
pages = "1158--1165",
journal = "Chemical Research in Toxicology",
issn = "0893-228X",
publisher = "American Chemical Society",
number = "12",

}

TY - JOUR

T1 - A highly toxic coplanar polychlorinated biphenyl compound suppresses Δ5 and Δ6 desaturase activities which play key roles in arachidonic acid synthesis in rat liver

AU - Matsusue, Kimihiko

AU - Ishii, Yuji

AU - Ariyoshi, Noritaka

AU - Oguri, Kazuta

PY - 1999

Y1 - 1999

N2 - The effect of 3,3',4,4',5-pentachlorobiphenyl (PenCB) on the synthesis of unsaturated fatty acids was studied in male Wistar rats. The arachidonic acid (20:4) content in the total lipids of liver homogenates was significantly reduced on day 5 of PenCB administration, while those of linoleic acid (18:2) and bishomo-γ-linolenic acid (20:3) were increased. These changes in the total lipids of liver homogenates were observed following doses of PenCB ranging from 0.5 to 25 mg/kg of body weight. The same changes in these fatty acids were seen with four subtypes of microsomal glycerophospholipids in the liver. The marked reduction in the molar ratio of 20:4 to 18:2 in the lipids suggests alteration of the activity of the enzymes responsible for the synthesis of unsaturated fatty acids. The activity of Δ5 and Δ6 desaturases (arachidonic acid synthetase) in the liver microsomes was 17 and 13% of that of pair-fed control animals, whereas the activity of 1- acylglycerophosphorylcholine or 1-acylglycerophosphate acyltransferase, which transfers 20:4 or 18:2 to phospholipids, was not affected by the treatment. Thus, the reduction in the level of 20:4 that was observed can be explained by a reduction in desaturase activity. These results are evidence that the coplanar PenCB has a significant effect on the reduced synthesis of physiologically essential long-chain unsaturated fatty acids.

AB - The effect of 3,3',4,4',5-pentachlorobiphenyl (PenCB) on the synthesis of unsaturated fatty acids was studied in male Wistar rats. The arachidonic acid (20:4) content in the total lipids of liver homogenates was significantly reduced on day 5 of PenCB administration, while those of linoleic acid (18:2) and bishomo-γ-linolenic acid (20:3) were increased. These changes in the total lipids of liver homogenates were observed following doses of PenCB ranging from 0.5 to 25 mg/kg of body weight. The same changes in these fatty acids were seen with four subtypes of microsomal glycerophospholipids in the liver. The marked reduction in the molar ratio of 20:4 to 18:2 in the lipids suggests alteration of the activity of the enzymes responsible for the synthesis of unsaturated fatty acids. The activity of Δ5 and Δ6 desaturases (arachidonic acid synthetase) in the liver microsomes was 17 and 13% of that of pair-fed control animals, whereas the activity of 1- acylglycerophosphorylcholine or 1-acylglycerophosphate acyltransferase, which transfers 20:4 or 18:2 to phospholipids, was not affected by the treatment. Thus, the reduction in the level of 20:4 that was observed can be explained by a reduction in desaturase activity. These results are evidence that the coplanar PenCB has a significant effect on the reduced synthesis of physiologically essential long-chain unsaturated fatty acids.

UR - http://www.scopus.com/inward/record.url?scp=0033397424&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033397424&partnerID=8YFLogxK

U2 - 10.1021/tx990104r

DO - 10.1021/tx990104r

M3 - Article

C2 - 10604864

AN - SCOPUS:0033397424

VL - 12

SP - 1158

EP - 1165

JO - Chemical Research in Toxicology

JF - Chemical Research in Toxicology

SN - 0893-228X

IS - 12

ER -