A hydrophilic polymer grafted with a histone tail peptide as an artificial gene regulator

Shujiro Shiosaki, Masanori Kuramoto, Riki Toita, Takeshi Mori, Takuro Niidome, Yoshiki Katayama

研究成果: ジャーナルへの寄稿記事

2 引用 (Scopus)

抄録

In chromatin, gene transcription is regulated through posttranslational modifications on the histone N-terminal tail sequences, typically an acetyl group modification on lysine residues. To realize a simple model of the gene regulation of chromatin, we designed a hydrophilic polymer grafted with histone H3 tail peptides. The polyplex formed from the polymer and DNA suppressed the gene expression effectively although the polyplex was weaker than the polyplex of poly-l-lysine and DNA. This weaker polyplex afforded the acetylation of the lysine residue of the grafted peptides by histone acetyltransferase. Subsequently, the gene expression was activated due to the relaxation of the polyplex which was brought by a cationic charge decrease in the grafted peptides. This molecular system is the first functional model of the gene regulation of the chromatin.

元の言語英語
ページ(範囲)4101-4105
ページ数5
ジャーナルBioorganic and Medicinal Chemistry
19
発行部数13
DOI
出版物ステータス出版済み - 7 1 2011

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Synthetic Genes
Gene expression
Histones
Chromatin
Polymers
Genes
Peptides
Lysine
Histone Acetyltransferases
Gene Expression
Post Translational Protein Processing
Acetylation
Transcription
DNA

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

これを引用

A hydrophilic polymer grafted with a histone tail peptide as an artificial gene regulator. / Shiosaki, Shujiro; Kuramoto, Masanori; Toita, Riki; Mori, Takeshi; Niidome, Takuro; Katayama, Yoshiki.

:: Bioorganic and Medicinal Chemistry, 巻 19, 番号 13, 01.07.2011, p. 4101-4105.

研究成果: ジャーナルへの寄稿記事

Shiosaki, Shujiro ; Kuramoto, Masanori ; Toita, Riki ; Mori, Takeshi ; Niidome, Takuro ; Katayama, Yoshiki. / A hydrophilic polymer grafted with a histone tail peptide as an artificial gene regulator. :: Bioorganic and Medicinal Chemistry. 2011 ; 巻 19, 番号 13. pp. 4101-4105.
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