TY - JOUR
T1 - A kinetic expression for hydrolysis of soluble starch by glucoamylase
AU - Kusunoki, Koichiro
AU - Kawakami, Koei
AU - Shiraishi, Fumihide
AU - Kato, Keiichi
AU - Kai, Mayumi
PY - 1982/2
Y1 - 1982/2
N2 - As the hydrolysis of starch by glucoamylase proceeds with stepwise removal of glucose units from the nonreducing ends of the starch chain, the number of available substrate molecules is essentially unchanged in the course of the degradation. In view of this aspect, a simple practical kinetic expression, which consists of a modified Michaelis–Menten form with product inhibition, is presented for the hydrolysis of soluble starch. It is assumed that the values of kinetic parameters Vm and Km vary linearly from the values for starch toward those for maltose. The applicability of this kinetic expression is verified through the simulation with the experimental results for the hydrolysis of two soluble starches with different average molecular weights of 3 × 104 and 3 × 106.
AB - As the hydrolysis of starch by glucoamylase proceeds with stepwise removal of glucose units from the nonreducing ends of the starch chain, the number of available substrate molecules is essentially unchanged in the course of the degradation. In view of this aspect, a simple practical kinetic expression, which consists of a modified Michaelis–Menten form with product inhibition, is presented for the hydrolysis of soluble starch. It is assumed that the values of kinetic parameters Vm and Km vary linearly from the values for starch toward those for maltose. The applicability of this kinetic expression is verified through the simulation with the experimental results for the hydrolysis of two soluble starches with different average molecular weights of 3 × 104 and 3 × 106.
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U2 - 10.1002/bit.260240208
DO - 10.1002/bit.260240208
M3 - Article
AN - SCOPUS:84988099033
VL - 24
SP - 347
EP - 354
JO - Biotechnology and Bioengineering
JF - Biotechnology and Bioengineering
SN - 0006-3592
IS - 2
ER -