A lipid bilayer formed on a hydrogel bead for single ion channel recordings

Minako Hirano, Daiki Yamamoto, Mami Asakura, Tohru Hayakawa, Shintaro Mise, Akinobu Matsumoto, Toru Ide

研究成果: Contribution to journalArticle査読

1 被引用数 (Scopus)

抄録

Ion channel proteins play important roles in various cell functions, making them attractive drug targets. Artificial lipid bilayer recording is a technique used to measure the ion transport activities of channel proteins with high sensitivity and accuracy. However, the measurement efficiency is low. In order to improve the efficiency, we developed a method that allows us to form bilayers on a hydrogel bead and record channel currents promptly. We tested our system by measuring the activities of various types of channels, including gramicidin, alamethicin, α-hemolysin, a voltage-dependent anion channel 1 (VDAC1), a voltage-and calcium-activated large conductance potassium channel (BK channel), and a potassium channel from Streptomyces lividans (KcsA channel). We confirmed the ability for enhanced measurement efficiency and measurement system miniaturizion.

本文言語英語
論文番号1070
ページ(範囲)1-15
ページ数15
ジャーナルMicromachines
11
12
DOI
出版ステータス出版済み - 12 2020

All Science Journal Classification (ASJC) codes

  • 制御およびシステム工学
  • 機械工学
  • 電子工学および電気工学

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