A luminol chemiluminescence method for sensing histidine and lysine using enzyme reactions

Akimitsu Kugimiya, Rie Fukada, Daiki Funamoto

研究成果: Contribution to journalArticle

14 引用 (Scopus)

抜粋

The analysis of free amino acids in urine and plasma is useful for estimating disease status in clinical diagnoses. Changes in the concentration of free amino acids in foods are also useful markers of freshness, nutrition, and taste. In this study, the specific interaction between aminoacyl-tRNA synthetase (aaRS) and its corresponding amino acid was used to measure amino acid concentrations. Pyrophosphate released by the amino acid-aaRS binding reaction was detected by luminol chemiluminescence; the method provided selective quantitation of 1.0-30 μM histidine and 1.0-60 μM lysine.

元の言語英語
ページ(範囲)22-26
ページ数5
ジャーナルAnalytical Biochemistry
443
発行部数1
DOI
出版物ステータス出版済み - 1 1 2013

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

フィンガープリント A luminol chemiluminescence method for sensing histidine and lysine using enzyme reactions' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用