A Lysosomal Protein Negatively Regulates Surface T Cell Antigen Receptor Expression by Promoting CD3ζ-Chain Degradation

Rika Ouchida, Sho Yamasaki, Masaki Hikida, Keiji Masuda, Kiyoko Kawamura, Akihiko Wada, Shigenobu Mochizuki, Masatoshi Tagawa, Akemi Sakamoto, Masahiko Hatano, Takeshi Tokuhisa, Haruhiko Koseki, Takashi Saito, Tomohiro Kurosaki, Ji Yang Wang

研究成果: Contribution to journalArticle査読

48 被引用数 (Scopus)

抄録

Modulation of surface T cell antigen receptor (TCR) expression is an important mechanism for the regulation of immune responses and the prevention of T cell hyperactivation and autoimmunity. The TCR is rapidly internalized after antigen stimulation and then degraded in lysosomes. However, few of the molecules involved in this process have been identified. We demonstrate that the lysosomal protein LAPTM5 negatively regulated surface TCR expression by specifically interacting with the invariant signal-transducing CD3ζ chain and promoting its degradation without affecting other CD3 proteins, CD3ε, CD3δ, or CD3γ. TCR downmodulation required the polyproline-tyrosine motifs and the ubiquitin-interacting motif of LAPTM5. LAPTM5 deficiency resulted in elevated TCR expression on both CD4+CD8+ thymocytes and spleen T cells after CD3 stimulation, as well as enhanced T cell responses in vitro and in vivo. These results identify a lysosomal protein important for CD3ζ degradation and illustrate a unique mechanism for the control of surface TCR expression and T cell activation.

本文言語英語
ページ(範囲)33-43
ページ数11
ジャーナルImmunity
29
1
DOI
出版ステータス出版済み - 7 18 2008

All Science Journal Classification (ASJC) codes

  • 免疫アレルギー学
  • 免疫学
  • 感染症

フィンガープリント

「A Lysosomal Protein Negatively Regulates Surface T Cell Antigen Receptor Expression by Promoting CD3ζ-Chain Degradation」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル