A measuring method for occupancy of immune checkpoint inhibitors in the cell surface

Toyoshi Yanagihara, Kentaro Tanaka, Koichiro Matsumoto

研究成果: Contribution to journalArticle査読

抄録

Monoclonal antibodies, including immune-checkpoint inhibitors, are becoming popular in treatments of many cancers and connective tissue diseases. However, little is known about how long the antibodies combine with antigens on targeted cells or how this duration of binding associates with therapeutic efficacy or potential adverse events. Here, we show the principle and the results of a feasible method for measuring the antibodies’ occupancy on the targeted cells using two different detecting antibodies in conjunction with different fluorochromes. Nivolumab occupancy was measured using two detecting antibodies, MIH4 and EH12.2, which are commercially available in vitro (programmed cell death-1 [PD-1] expressing the cell line MIT9 and human T cells) and in T cells from patients treated with nivolumab. Our method has potential for use as a simple and feasible monitoring system in the clinical setting.

本文言語英語
ページ(範囲)213-217
ページ数5
ジャーナルBiochemical and Biophysical Research Communications
527
1
DOI
出版ステータス出版済み - 6 18 2020

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

フィンガープリント

「A measuring method for occupancy of immune checkpoint inhibitors in the cell surface」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル