A mechanism of transcriptional regulation of the CSF-1 gene by interferon-gamma

Daisuke Tsuchimoto, Arinobu Tojo, Shigetaka Asano

研究成果: Contribution to journalArticle査読

7 被引用数 (Scopus)

抄録

Interferon (IFN)-gamma exerts multiple functions including antiviral, anti-proliferative and immunomodulatory activities, which are mediated through the JAK-STAT pathway. We observed that IFN-gamma significantly increases the production of colony stimulating factor-1 (CSF-1) by a human lung carcinoma cell line, A549. To gain insight into this mechanism, we determined the unknown nucleotide sequences of 5′-flanking region of human CSF-1 gene. About 60 bases upstream of the transcription start site of the CSF-1 gene contains a possible gamma interferon activated site (GAS), TTCCCATAA. The promoter assay and the electrophoretic mobility gel shift assay revealed that IFN-gamma stimulates transcription of the CSF-1 gene through this sequence, which binds STAT1.

本文言語英語
ページ(範囲)397-405
ページ数9
ジャーナルImmunological Investigations
33
4
DOI
出版ステータス出版済み - 2004

All Science Journal Classification (ASJC) codes

  • 免疫学

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