A membrane protein, TMCO5A, has a close relationship with manchette microtubules in rat spermatids during spermiogenesis

Takane Kaneko, Taisuke Minohara, Sakurako Shima, Kaori Yoshida, Atsuko Fukuda, Naoki Iwamori, Tetsuichiro Inai, Hiroshi Iida

研究成果: ジャーナルへの寄稿記事

抄録

We isolated the transmembrane and coiled-coil domains 5A (Tmco5A) gene using polymerase chain reaction-based subtraction technique and showed that Tmco5A was predominantly expressed in rat testes starting at 4 weeks of postnatal development. When expressed in COS7 cells, TMCO5A was found to be distributed in the endoplasmic reticulum-nuclear membrane (ER-NM) of cells as a membrane-associated protein, while TMCO5AΔC lacking the transmembrane region (TM) mislocalized and diffused throughout the cytoplasm. The result suggested that TM is responsible for the retention of TMCO5A at the ER-NM. Immunocytochemical and immunoblotting analyses indicated that TMCO5A was localized along the posterior part of the nuclei in both round and elongated rat spermatids but disappeared from epididymal spermatozoa. Double immunolabeling of isolated spermatids with the anti-TMCO5A and the anti-β tubulin antibodies showed that TMCO5A was always found to be closely associated with developing manchette microtubules but did not completely colocalize with them. On the other hand, we found that almost all TMCO5A colocalized with SUN4, a linker of nucleoskeleton and cytoskeleton complex protein present at the posterior part of spermatid nuclei. These data suggested that TMCO5A is located closer to the nuclei than the manchette microtubules. It is likely that TMCO5A, in association with manchette microtubules, is involved in the process of spermiogenesis.

元の言語英語
ページ(範囲)330-341
ページ数12
ジャーナルMolecular Reproduction and Development
86
発行部数3
DOI
出版物ステータス出版済み - 3 1 2019

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Spermatids
Spermatogenesis
Microtubules
Membrane Proteins
Nuclear Envelope
Endoplasmic Reticulum
Subtraction Technique
Nuclear Matrix
Tubulin
Cytoskeleton
Immunoblotting
Spermatozoa
Testis
Anti-Idiotypic Antibodies
Cytoplasm
Polymerase Chain Reaction
Genes
Proteins

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology
  • Cell Biology

これを引用

A membrane protein, TMCO5A, has a close relationship with manchette microtubules in rat spermatids during spermiogenesis. / Kaneko, Takane; Minohara, Taisuke; Shima, Sakurako; Yoshida, Kaori; Fukuda, Atsuko; Iwamori, Naoki; Inai, Tetsuichiro; Iida, Hiroshi.

:: Molecular Reproduction and Development, 巻 86, 番号 3, 01.03.2019, p. 330-341.

研究成果: ジャーナルへの寄稿記事

Kaneko, Takane ; Minohara, Taisuke ; Shima, Sakurako ; Yoshida, Kaori ; Fukuda, Atsuko ; Iwamori, Naoki ; Inai, Tetsuichiro ; Iida, Hiroshi. / A membrane protein, TMCO5A, has a close relationship with manchette microtubules in rat spermatids during spermiogenesis. :: Molecular Reproduction and Development. 2019 ; 巻 86, 番号 3. pp. 330-341.
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abstract = "We isolated the transmembrane and coiled-coil domains 5A (Tmco5A) gene using polymerase chain reaction-based subtraction technique and showed that Tmco5A was predominantly expressed in rat testes starting at 4 weeks of postnatal development. When expressed in COS7 cells, TMCO5A was found to be distributed in the endoplasmic reticulum-nuclear membrane (ER-NM) of cells as a membrane-associated protein, while TMCO5AΔC lacking the transmembrane region (TM) mislocalized and diffused throughout the cytoplasm. The result suggested that TM is responsible for the retention of TMCO5A at the ER-NM. Immunocytochemical and immunoblotting analyses indicated that TMCO5A was localized along the posterior part of the nuclei in both round and elongated rat spermatids but disappeared from epididymal spermatozoa. Double immunolabeling of isolated spermatids with the anti-TMCO5A and the anti-β tubulin antibodies showed that TMCO5A was always found to be closely associated with developing manchette microtubules but did not completely colocalize with them. On the other hand, we found that almost all TMCO5A colocalized with SUN4, a linker of nucleoskeleton and cytoskeleton complex protein present at the posterior part of spermatid nuclei. These data suggested that TMCO5A is located closer to the nuclei than the manchette microtubules. It is likely that TMCO5A, in association with manchette microtubules, is involved in the process of spermiogenesis.",
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T1 - A membrane protein, TMCO5A, has a close relationship with manchette microtubules in rat spermatids during spermiogenesis

AU - Kaneko, Takane

AU - Minohara, Taisuke

AU - Shima, Sakurako

AU - Yoshida, Kaori

AU - Fukuda, Atsuko

AU - Iwamori, Naoki

AU - Inai, Tetsuichiro

AU - Iida, Hiroshi

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