A mouse B16 melanoma mutant deficient in glycolipids

Shinichi Ichikawa, Nobushige Nakajo, Hisako Sakiyama, Yoshio Hirabayashi

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Mouse B16 melanoma cell line, GM-95 (formerly designated as MEC-4), deficient in sialyllactosylceramide was examined for its primary defect. Glycolipids from the mutant cells were analyzed by high-performance TLC. No glycolipid was detected in GM-95 cells, even when total lipid from 107 cells was analyzed. In contrast, the content of ceramide, a precursor lipid molecule of glycolipids, was normal. Thus, the deficiency of glycolipids was attributed to the first glucosylation step of ceramide. The ceramide glucosyltransferase (EC 2.4.1.80) activity was not detected in GM-95 cells. There was no significant difference of sialyllactosylceramide synthase activity, however, between GM-95 and the parental cells. The deficiency of glycolipids in GM-95 cells was associated with changes of the cellular morphology and growth rate. The parental cells showed irregular shapes and tended to overlap each other. On the other hand, GM-95 cells exhibited an elongated fibroblastic morphology and parallel arrangement. The population- doubling times of GM-95 and the parental cells in serum-free medium were 28 hr and 19 hr, respectively.

元の言語英語
ページ(範囲)2703-2707
ページ数5
ジャーナルProceedings of the National Academy of Sciences of the United States of America
91
発行部数7
DOI
出版物ステータス出版済み - 3 29 1994

All Science Journal Classification (ASJC) codes

  • General

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