A murC gene in porphyromonas gingivalis 381

T. Ansai, Yoshihisa Yamashita, S. Awano, Yukie Shibata, M. Wachi, K. Nagai, T. Takehara

研究成果: ジャーナルへの寄稿記事

14 引用 (Scopus)

抄録

The gene encoding a 51 kDa polypeptide of Porphyromonas gingivalis 381 was isolated by immunoblotting using an antiserum raised against P. gingivalis alkaline phosphatase. DNA sequence analysis of a 2.5 kb DNA fragment containing a gene encoding the 51 kDa protein revealed one complete and two incomplete ORFs. Database searches using the FASTA program revealed significant homology between the P. gingivalis 51 kDa protein and the MurC protein of Escherichia coli, which functions in peptidoglycan synthesis. The cloned 51 kDa protein encoded a functional product that complemented an E. coli murC mutant. Moreover, the ORF just upstream of murC coded for a Institute of Technology, protein that was 31% homologous with the E. coli MurG protein. The ORF just downstream of murC coded for a protein that was 17% homologous with the Streptococcus pneumoniae penicillin-binding protein 2B (PBP2B), which functions in peptidoglycan synthesis and is responsible for antibiotic resistance. These results suggest that P. gingivalis contains a homologue of the E. coil peptidoglycan synthesis gene murC and indicate the possibility of a cluster of genes responsible for cell division and cell growth, as in the E. coil mra region.

元の言語英語
ページ(範囲)2047-2052
ページ数6
ジャーナルMicrobiology
141
発行部数9
DOI
出版物ステータス出版済み - 1 1 1995
外部発表Yes

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Porphyromonas gingivalis
Peptidoglycan
Open Reading Frames
Genes
Escherichia coli Proteins
Proteins
Multigene Family
Microbial Drug Resistance
Streptococcus pneumoniae
DNA Sequence Analysis
Immunoblotting
Cell Division
Alkaline Phosphatase
Immune Sera
Databases
Escherichia coli
Technology
Peptides
DNA
Growth

All Science Journal Classification (ASJC) codes

  • Microbiology

これを引用

Ansai, T., Yamashita, Y., Awano, S., Shibata, Y., Wachi, M., Nagai, K., & Takehara, T. (1995). A murC gene in porphyromonas gingivalis 381. Microbiology, 141(9), 2047-2052. https://doi.org/10.1099/13500872-141-9-2047

A murC gene in porphyromonas gingivalis 381. / Ansai, T.; Yamashita, Yoshihisa; Awano, S.; Shibata, Yukie; Wachi, M.; Nagai, K.; Takehara, T.

:: Microbiology, 巻 141, 番号 9, 01.01.1995, p. 2047-2052.

研究成果: ジャーナルへの寄稿記事

Ansai, T, Yamashita, Y, Awano, S, Shibata, Y, Wachi, M, Nagai, K & Takehara, T 1995, 'A murC gene in porphyromonas gingivalis 381', Microbiology, 巻. 141, 番号 9, pp. 2047-2052. https://doi.org/10.1099/13500872-141-9-2047
Ansai, T. ; Yamashita, Yoshihisa ; Awano, S. ; Shibata, Yukie ; Wachi, M. ; Nagai, K. ; Takehara, T. / A murC gene in porphyromonas gingivalis 381. :: Microbiology. 1995 ; 巻 141, 番号 9. pp. 2047-2052.
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