A mutant rat strain defficient in induction of a phenobarbital-inducible form of cytochrome P-450 in liver microsomes

Toshihide Hashimoto, Tatsumi Matsumoto, Mayumi Nishizawa, Shun-Ichiro Kawabata, Ken-Ichirou Morohashi, Sumio Handa, Tsuneo Omura

研究成果: ジャーナルへの寄稿記事

18 引用 (Scopus)

抄録

Two phenobarbital-inducible forms of cytochrome P-450, P-450(PB-1), and P-450(PB-4), were purified from the liver microsomes of phenobarbital-treated rats and identified with P-450b and P-450e, respectively. It was found, however, that the content of P-450(PB-4) in the liver microsomes of a strain of SD rat, Qdj: SD, was very low even after phenobarbital-induction. The levels of the mRNAs for P-450(PB-1) and P-450(PB-4) were separately determined using Northern blot hybridization with specific oligonucleotide probes. It was found that the level of P-450(PB-4) mRNA in the livers of phenobarbital-treated Qdj: SD rats was much lower than that of phenobarbital-treated Slc: SD rats. Slc:SD rats are widely used in Japanese laboratories. Genetic analysis using the crossbred animals between Qdj: SD and Slc: SD rats showed that the low expression of P-450(PB-4) in Qdj: SD rats is a recessive trait and is caused by a single gene mutation. However, no difference in the 5' flanking region in P-450(PB-4) gene was found between Qdj: SD rats and Slc: SD rats.

元の言語英語
ページ(範囲)487-492
ページ数6
ジャーナルJournal of Biochemistry
103
発行部数3
DOI
出版物ステータス出版済み - 1 1 1988

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Mutant Strains Rats
Liver Microsomes
Phenobarbital
Liver
Cytochrome P-450 Enzyme System
Rats
Genes
Messenger RNA
Oligonucleotide Probes
5' Flanking Region
Northern Blotting
Animals

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

これを引用

A mutant rat strain defficient in induction of a phenobarbital-inducible form of cytochrome P-450 in liver microsomes. / Hashimoto, Toshihide; Matsumoto, Tatsumi; Nishizawa, Mayumi; Kawabata, Shun-Ichiro; Morohashi, Ken-Ichirou; Handa, Sumio; Omura, Tsuneo.

:: Journal of Biochemistry, 巻 103, 番号 3, 01.01.1988, p. 487-492.

研究成果: ジャーナルへの寄稿記事

Hashimoto, Toshihide ; Matsumoto, Tatsumi ; Nishizawa, Mayumi ; Kawabata, Shun-Ichiro ; Morohashi, Ken-Ichirou ; Handa, Sumio ; Omura, Tsuneo. / A mutant rat strain defficient in induction of a phenobarbital-inducible form of cytochrome P-450 in liver microsomes. :: Journal of Biochemistry. 1988 ; 巻 103, 番号 3. pp. 487-492.
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abstract = "Two phenobarbital-inducible forms of cytochrome P-450, P-450(PB-1), and P-450(PB-4), were purified from the liver microsomes of phenobarbital-treated rats and identified with P-450b and P-450e, respectively. It was found, however, that the content of P-450(PB-4) in the liver microsomes of a strain of SD rat, Qdj: SD, was very low even after phenobarbital-induction. The levels of the mRNAs for P-450(PB-1) and P-450(PB-4) were separately determined using Northern blot hybridization with specific oligonucleotide probes. It was found that the level of P-450(PB-4) mRNA in the livers of phenobarbital-treated Qdj: SD rats was much lower than that of phenobarbital-treated Slc: SD rats. Slc:SD rats are widely used in Japanese laboratories. Genetic analysis using the crossbred animals between Qdj: SD and Slc: SD rats showed that the low expression of P-450(PB-4) in Qdj: SD rats is a recessive trait and is caused by a single gene mutation. However, no difference in the 5' flanking region in P-450(PB-4) gene was found between Qdj: SD rats and Slc: SD rats.",
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