抄録
Two phenobarbital-inducible forms of cytochrome P-450, P-450(PB-1), and P-450(PB-4), were purified from the liver microsomes of phenobarbital-treated rats and identified with P-450b and P-450e, respectively. It was found, however, that the content of P-450(PB-4) in the liver microsomes of a strain of SD rat, Qdj: SD, was very low even after phenobarbital-induction. The levels of the mRNAs for P-450(PB-1) and P-450(PB-4) were separately determined using Northern blot hybridization with specific oligonucleotide probes. It was found that the level of P-450(PB-4) mRNA in the livers of phenobarbital-treated Qdj: SD rats was much lower than that of phenobarbital-treated Slc: SD rats. Slc:SD rats are widely used in Japanese laboratories. Genetic analysis using the crossbred animals between Qdj: SD and Slc: SD rats showed that the low expression of P-450(PB-4) in Qdj: SD rats is a recessive trait and is caused by a single gene mutation. However, no difference in the 5' flanking region in P-450(PB-4) gene was found between Qdj: SD rats and Slc: SD rats.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 487-492 |
| ページ数 | 6 |
| ジャーナル | Journal of Biochemistry |
| 巻 | 103 |
| 発行部数 | 3 |
| DOI | |
| 出版物ステータス | 出版済み - 1 1 1988 |
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All Science Journal Classification (ASJC) codes
- Biochemistry
- Molecular Biology
これを引用
A mutant rat strain defficient in induction of a phenobarbital-inducible form of cytochrome P-450 in liver microsomes. / Hashimoto, Toshihide; Matsumoto, Tatsumi; Nishizawa, Mayumi; Kawabata, Shun-Ichiro; Morohashi, Ken-Ichirou; Handa, Sumio; Omura, Tsuneo.
：: Journal of Biochemistry, 巻 103, 番号 3, 01.01.1988, p. 487-492.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - A mutant rat strain defficient in induction of a phenobarbital-inducible form of cytochrome P-450 in liver microsomes
AU - Hashimoto, Toshihide
AU - Matsumoto, Tatsumi
AU - Nishizawa, Mayumi
AU - Kawabata, Shun-Ichiro
AU - Morohashi, Ken-Ichirou
AU - Handa, Sumio
AU - Omura, Tsuneo
PY - 1988/1/1
Y1 - 1988/1/1
N2 - Two phenobarbital-inducible forms of cytochrome P-450, P-450(PB-1), and P-450(PB-4), were purified from the liver microsomes of phenobarbital-treated rats and identified with P-450b and P-450e, respectively. It was found, however, that the content of P-450(PB-4) in the liver microsomes of a strain of SD rat, Qdj: SD, was very low even after phenobarbital-induction. The levels of the mRNAs for P-450(PB-1) and P-450(PB-4) were separately determined using Northern blot hybridization with specific oligonucleotide probes. It was found that the level of P-450(PB-4) mRNA in the livers of phenobarbital-treated Qdj: SD rats was much lower than that of phenobarbital-treated Slc: SD rats. Slc:SD rats are widely used in Japanese laboratories. Genetic analysis using the crossbred animals between Qdj: SD and Slc: SD rats showed that the low expression of P-450(PB-4) in Qdj: SD rats is a recessive trait and is caused by a single gene mutation. However, no difference in the 5' flanking region in P-450(PB-4) gene was found between Qdj: SD rats and Slc: SD rats.
AB - Two phenobarbital-inducible forms of cytochrome P-450, P-450(PB-1), and P-450(PB-4), were purified from the liver microsomes of phenobarbital-treated rats and identified with P-450b and P-450e, respectively. It was found, however, that the content of P-450(PB-4) in the liver microsomes of a strain of SD rat, Qdj: SD, was very low even after phenobarbital-induction. The levels of the mRNAs for P-450(PB-1) and P-450(PB-4) were separately determined using Northern blot hybridization with specific oligonucleotide probes. It was found that the level of P-450(PB-4) mRNA in the livers of phenobarbital-treated Qdj: SD rats was much lower than that of phenobarbital-treated Slc: SD rats. Slc:SD rats are widely used in Japanese laboratories. Genetic analysis using the crossbred animals between Qdj: SD and Slc: SD rats showed that the low expression of P-450(PB-4) in Qdj: SD rats is a recessive trait and is caused by a single gene mutation. However, no difference in the 5' flanking region in P-450(PB-4) gene was found between Qdj: SD rats and Slc: SD rats.
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UR - http://www.scopus.com/inward/citedby.url?scp=0023835238&partnerID=8YFLogxK
U2 - 10.1093/oxfordjournals.jbchem.a122297
DO - 10.1093/oxfordjournals.jbchem.a122297
M3 - Article
C2 - 2839467
AN - SCOPUS:0023835238
VL - 103
SP - 487
EP - 492
JO - Journal of Biochemistry
JF - Journal of Biochemistry
SN - 0021-924X
IS - 3
ER -