A mutation study of catalytic residue Asp 52 in hen egg lysozyme

Yoshio Hashimoto, Keiko Yamada, Hiroyuki Motoshima, Tadahiro Omura, Hidenori Yamada, Takanori Yasukochi, Takeyoshi Miki, Tadashi Ueda, Taiji Imoto

研究成果: Contribution to journalArticle査読

45 被引用数 (Scopus)

抄録

We constructed a system for the expression and secretion of mature hen lysozyme by yeast using an intermediate 'secretion-signal cassette' vector, pKP1700, containing the yeast invertase signal sequence and an expression vector, pAM82, for secretion and maturation of the enzyme. Using this system, mutants of hen lysozyme were produced and the catalytic mechanism in hen lysozyme was definitely confirmed. The hydrolytic activity of D52A as to substrate (NAG), at pH 5.0 was obviously decreased to one-four hundredth of that of the wild type. The acidic limb of the pH-activity profile observed for the wild-type was not observed for D52A, and the pK(a) of Glu 35 on the alkaline limb was seen for both enzymes. Moreover, no structural change was detected on X-ray analysis of D52A. Therefore, we confirmed that dissociated Asp 52 assists catalysis by producing an electrostatic field and by stabilizing the oxocarbonium ion intermediate in the dissociated form.

本文言語英語
ページ(範囲)145-150
ページ数6
ジャーナルJournal of biochemistry
119
1
DOI
出版ステータス出版済み - 1 1996

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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