The musculoskeletal system is a unique combination of soft and hard tissues in the body, consisted of bone, cartilage, muscle and tendon. These skeletal tissue formation are in essence organized by the tissue specific cells, including osteoblasts, osteocytes, chondrocytes, myocytes and fibroblasts. Mechanical stress has a variety of effects on the structure and function of their cells. However, little is known about the effect of mechanical stress on cell differentiation regarding the quality and quantity of the stress and the timing applied. In the present study, we developed a culture device that enables to load the mechanical stress quantitatively with ease to evaluate proliferation and differentiation of cells.