A new exploration in enhancing the resolutionfor high-performance analysis of mono-sulfate glycosaminoglycan disaccharide isomers on a poly(methyl methacrylate) chip

Yong Zhang, Guichen Ping, Bingmei Zhu, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba

研究成果: 会議への寄与タイプ学会誌査読

抄録

The glycosaminoglycans (GAGs) labeled with 2-aminoacridone, hydrochloride (AMAC) were found to be prevented from adsorption to the surface of polymehylmethacrylate (PMMA) microchips by dynamic coating microchannel with methyl cellulose (MC). To obtain sufficient resolution of mono-sulfate GAG disaccharide isomers on microchip electrophoresis (MCE), buffer pH, β-cyclodextrin (β-CD), and 1,4-dioxane (DO) were systematically investigated. We found that the application of 1,4-dioxane (DO) dramatically improved the resolution of the analytes. ΔDi-triS, ΔDi-UA2S, ΔDi-4S, and ΔDi-6S were baseline separated within 75 sec. The theoretical plate was in the range of 5×105-1×10 6 m-1.

本文言語英語
ページ395-397
ページ数3
出版ステータス出版済み - 2006
外部発表はい
イベント10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, 日本
継続期間: 11月 5 200611月 9 2006

その他

その他10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
国/地域日本
CityTokyo
Period11/5/0611/9/06

!!!All Science Journal Classification (ASJC) codes

  • 化学工学(その他)
  • バイオエンジニアリング

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