A new mib allele with a chromosomal deletion covering foxc1a exhibits anterior somite specification defect

Chia Hao Hsu, Ji Sheng Lin, Keng Po Lai, Jing Woei Li, Ting Fung Chan, May Su You, William Ka Fai Tse, Yun Jin Jiang

研究成果: ジャーナルへの寄稿記事

6 引用 (Scopus)

抄録

mib nn2002, found from an allele screen, showed early segmentation defect and severe cell death phenotypes, which are different from previously known mib mutants. Despite distinct morphological phenotypes, the typical mib molecular phenotypes: her4 down-regulation, neurogenic phenotype and cold sensitive dlc expression pattern, still remained. The linkage analysis also indicated that mib nn2002 is a new mib allele. Failure of specification in anterior 7-10 somites is likely due to lack of foxc1a expression in mib nn2002 homozygotes. Somites and somite markers gradually appeared after 7-10 somite stage, suggesting that foxc1a is only essential for the formation of anterior 7-10 somites. Apoptosis began around 16-somite stage with p53 up-regulation. To find the possible links of mib, foxc1a and apoptosis, transcriptome analysis was employed. About 140 genes, including wnt3a, foxc1a and mib, were not detected in the homozygotes. Overexpression of foxc1a mRNA in mib nn2002 homozygotes partially rescued the anterior somite specification. In the process of characterizing mib nn2002 mutation, we integrated the scaffolds containing mib locus into chromosome 2 (or linkage group 2, LG2) based on synteny comparison and transcriptome results. Genomic PCR analysis further supported the conclusion and showed that mib nn2002 has a chromosomal deletion with the size of about 9.6 Mbp.

元の言語英語
記事番号10673
ジャーナルScientific reports
5
DOI
出版物ステータス出版済み - 6 3 2015

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Somites
Alleles
Homozygote
Phenotype
Apoptosis
Synteny
Chromosomes, Human, Pair 2
Gene Expression Profiling
Transcriptome
Cell Death
Up-Regulation
Down-Regulation
Polymerase Chain Reaction
Messenger RNA
Mutation
Genes

All Science Journal Classification (ASJC) codes

  • General

これを引用

Hsu, C. H., Lin, J. S., Po Lai, K., Li, J. W., Chan, T. F., You, M. S., ... Jiang, Y. J. (2015). A new mib allele with a chromosomal deletion covering foxc1a exhibits anterior somite specification defect. Scientific reports, 5, [10673]. https://doi.org/10.1038/srep10673

A new mib allele with a chromosomal deletion covering foxc1a exhibits anterior somite specification defect. / Hsu, Chia Hao; Lin, Ji Sheng; Po Lai, Keng; Li, Jing Woei; Chan, Ting Fung; You, May Su; Tse, William Ka Fai; Jiang, Yun Jin.

:: Scientific reports, 巻 5, 10673, 03.06.2015.

研究成果: ジャーナルへの寄稿記事

Hsu, Chia Hao ; Lin, Ji Sheng ; Po Lai, Keng ; Li, Jing Woei ; Chan, Ting Fung ; You, May Su ; Tse, William Ka Fai ; Jiang, Yun Jin. / A new mib allele with a chromosomal deletion covering foxc1a exhibits anterior somite specification defect. :: Scientific reports. 2015 ; 巻 5.
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abstract = "mib nn2002, found from an allele screen, showed early segmentation defect and severe cell death phenotypes, which are different from previously known mib mutants. Despite distinct morphological phenotypes, the typical mib molecular phenotypes: her4 down-regulation, neurogenic phenotype and cold sensitive dlc expression pattern, still remained. The linkage analysis also indicated that mib nn2002 is a new mib allele. Failure of specification in anterior 7-10 somites is likely due to lack of foxc1a expression in mib nn2002 homozygotes. Somites and somite markers gradually appeared after 7-10 somite stage, suggesting that foxc1a is only essential for the formation of anterior 7-10 somites. Apoptosis began around 16-somite stage with p53 up-regulation. To find the possible links of mib, foxc1a and apoptosis, transcriptome analysis was employed. About 140 genes, including wnt3a, foxc1a and mib, were not detected in the homozygotes. Overexpression of foxc1a mRNA in mib nn2002 homozygotes partially rescued the anterior somite specification. In the process of characterizing mib nn2002 mutation, we integrated the scaffolds containing mib locus into chromosome 2 (or linkage group 2, LG2) based on synteny comparison and transcriptome results. Genomic PCR analysis further supported the conclusion and showed that mib nn2002 has a chromosomal deletion with the size of about 9.6 Mbp.",
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AU - Chan, Ting Fung

AU - You, May Su

AU - Tse, William Ka Fai

AU - Jiang, Yun Jin

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