A new odorless procedure for the synthesis of 2'-deoxy-6-thioguanosine and its incorporation into oligodeoxynucleotides

Kazumitsu Onizuka, Yosuke Taniguchi, Shigeki Sasaki

研究成果: Contribution to journalArticle査読

7 被引用数 (Scopus)

抄録

6-S-[2-[(2-ethylhexyl)oxycarbonyl]ethyl)}-3',5'-O-bis(tert- butyldimethylsilyl)-2'-deoxy-6-thiogua nosine (2) was synthesized in high yield from the corresponding 6-O-mesitylenesulfonyl derivative by the reaction with 2-ethylhexyl 3-mercapto-propionate. The phosphoramidite precursor derived from 2 was successfully applied to an automated DNA synthesizer to produce 2'-deoxy-6-thioguanosine containing ODN. The results showed that 2-ethylhexyl 3-mercaptopropionate is useful as an odor less reagent and also as an S-protecting group of 2'-deoxy-6-thioguanosine.

本文言語英語
ページ(範囲)752-760
ページ数9
ジャーナルNucleosides, Nucleotides and Nucleic Acids
28
8
DOI
出版ステータス出版済み - 8 2009

All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子医療
  • 遺伝学

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