A New Sulfonated Tetrazolium Salt That Produces a Highly Water-Soluble Formazan Dye

Munetaka Ishiyama, Masanobu Shiga, Kazumi Sasamoto, Makoto Mizoguchi, Pingang He

研究成果: ジャーナルへの寄稿記事

435 引用 (Scopus)

抄録

A new tetrazolium compound, sodium salt of 4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-l,3-benzene disulfonate (la), which produces a highly water-soluble formazan dye, due to the presence of two sulfonate groups, was synthesized and its potential utility evaluated in assays of NADH and cell proliferation. The compound proved to have a similar sensitivity to 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide (XTT) in the assay of NADH and was also useful as an indicator of cell viability, with less cytotoxicity than XTT, in the proliferation assay using P388 cell lines.

元の言語英語
ページ(範囲)1118-1122
ページ数5
ジャーナルChemical and Pharmaceutical Bulletin
41
発行部数6
DOI
出版物ステータス出版済み - 1 1 1993

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Tetrazolium Salts
Formazans
NAD
Assays
Coloring Agents
Sodium Compounds
Water
Benzene
Cells
Cell Survival
Salts
Cell Proliferation
Cell proliferation
Cytotoxicity
Cell Line
4-nitrophenyl
2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Drug Discovery

これを引用

A New Sulfonated Tetrazolium Salt That Produces a Highly Water-Soluble Formazan Dye. / Ishiyama, Munetaka; Shiga, Masanobu; Sasamoto, Kazumi; Mizoguchi, Makoto; He, Pingang.

:: Chemical and Pharmaceutical Bulletin, 巻 41, 番号 6, 01.01.1993, p. 1118-1122.

研究成果: ジャーナルへの寄稿記事

Ishiyama, Munetaka ; Shiga, Masanobu ; Sasamoto, Kazumi ; Mizoguchi, Makoto ; He, Pingang. / A New Sulfonated Tetrazolium Salt That Produces a Highly Water-Soluble Formazan Dye. :: Chemical and Pharmaceutical Bulletin. 1993 ; 巻 41, 番号 6. pp. 1118-1122.
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AU - He, Pingang

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