A new targeted capture method using bacterial artificial chromosome (BAC) libraries as baits for sequencing relatively large genes

Kae Koganebuchi, Takashi Gakuhari, Hirohiko Takeshima, Kimitoshi Sato, Kiyotaka Fujii, Toshihiro Kumabe, Satoshi Kasagi, Takehiro Sato, Atsushi Tajima, Hiroki Shibata, Motoyuki Ogawa, Hiroki Oota

研究成果: ジャーナルへの寄稿記事

抄録

To analyze a specific genome region using next-generation sequencing technologies, the enrichment of DNA libraries with targeted capture methods has been standardized. For enrichment of mitochondrial genome, a previous study developed an original targeted capture method that use baits constructed from long-range polymerase chain reaction (PCR) amplicons, common laboratory reagents, and equipment. In this study, a new targeted capture method is presented, that of bacterial artificial chromosome (BAC) double capture (BDC), modifying the previous method, but using BAC libraries as baits for sequencing a relatively large gene. We applied the BDC approach for the 214 kb autosomal region, ring finger protein 213, which is the susceptibility gene of moyamoya disease (MMD). To evaluate the reliability of BDC, cost and data quality were compared with those of a commercial kit. While the ratio of duplicate reads was higher, the cost was less than that of the commercial kit. The data quality was sufficiently the same as that of the kit. Thus, BDC can be an easy, low-cost, and useful method for analyzing individual genome regions with substantial length.

元の言語英語
記事番号e0200170
ジャーナルPloS one
13
発行部数7
DOI
出版物ステータス出版済み - 7 2018

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Bacterial Artificial Chromosomes
bacterial artificial chromosomes
Chromosomes
baits
Genes
genes
Costs and Cost Analysis
Genome
Moyamoya Disease
Costs
methodology
Mitochondrial Genome
genome
DNA libraries
Polymerase chain reaction
Gene Library
Fingers
polymerase chain reaction
Technology
Equipment and Supplies

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

これを引用

Koganebuchi, K., Gakuhari, T., Takeshima, H., Sato, K., Fujii, K., Kumabe, T., ... Oota, H. (2018). A new targeted capture method using bacterial artificial chromosome (BAC) libraries as baits for sequencing relatively large genes. PloS one, 13(7), [e0200170]. https://doi.org/10.1371/journal.pone.0200170

A new targeted capture method using bacterial artificial chromosome (BAC) libraries as baits for sequencing relatively large genes. / Koganebuchi, Kae; Gakuhari, Takashi; Takeshima, Hirohiko; Sato, Kimitoshi; Fujii, Kiyotaka; Kumabe, Toshihiro; Kasagi, Satoshi; Sato, Takehiro; Tajima, Atsushi; Shibata, Hiroki; Ogawa, Motoyuki; Oota, Hiroki.

:: PloS one, 巻 13, 番号 7, e0200170, 07.2018.

研究成果: ジャーナルへの寄稿記事

Koganebuchi, K, Gakuhari, T, Takeshima, H, Sato, K, Fujii, K, Kumabe, T, Kasagi, S, Sato, T, Tajima, A, Shibata, H, Ogawa, M & Oota, H 2018, 'A new targeted capture method using bacterial artificial chromosome (BAC) libraries as baits for sequencing relatively large genes', PloS one, 巻. 13, 番号 7, e0200170. https://doi.org/10.1371/journal.pone.0200170
Koganebuchi, Kae ; Gakuhari, Takashi ; Takeshima, Hirohiko ; Sato, Kimitoshi ; Fujii, Kiyotaka ; Kumabe, Toshihiro ; Kasagi, Satoshi ; Sato, Takehiro ; Tajima, Atsushi ; Shibata, Hiroki ; Ogawa, Motoyuki ; Oota, Hiroki. / A new targeted capture method using bacterial artificial chromosome (BAC) libraries as baits for sequencing relatively large genes. :: PloS one. 2018 ; 巻 13, 番号 7.
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