A new trisaccharide sugar chain linked to a serine residue in bovine blood coagulation factors VII and IX

Sumihiro Hase, Shun-Ichiro Kawabata, Hitoshi Nishimura, Hiroyuki Takeya, Tatsuya Sueyoshi, Toshiyuki Miyata, Sadaaki Iwanaga, Toshifumi Takao, Yasutsugu Shimonishi, Tokuji Ikenaka

研究成果: ジャーナルへの寄稿記事

82 引用 (Scopus)

抄録

A new trisaccharide sugar chain was identified in bovine blood coagulation factors VII and IX. A pentapeptide isolated from factor VII contained Ser-52, which could not be identified with a gas-phase sequencer, suggesting an unknown substituent on the serine residue (Takeya, H. et al. (1988) J. Biol. Chem., in press). The same results were obtained for a pentapeptide containing Ser-53 of factor IX. Component sugar analysis revealed that the peptide contained 1 mol of glucose and 2 mol of xylose. This sugar component was also confirmed by high-resolution fast atom bombardment mass spectrometric analysis of the pentapeptide. The trisaccharide was released from the peptides by means of β-elimination reaction and its reducing end was coupled with 2-aminopyridine. The fluorescent pyridylamino (PA-) derivative of the trisaccharide was purified by gel-filtration and reversed-phase HPLC. The sugar composition of the PA-trisaccharide was found to be 2 mol of xylose and 1 mol of PA-glucose. These results indicate the existence of a (Xyl2)Glc-Ser structure in factors VII and IX.

元の言語英語
ページ(範囲)867-868
ページ数2
ジャーナルJournal of Biochemistry
104
発行部数6
DOI
出版物ステータス出版済み - 1 1 1988

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Trisaccharides
Factor VII
Factor IX
Sugars
Serine
Xylose
Glucose
Peptides
Gel Chromatography
Gases
Gels
High Pressure Liquid Chromatography
Derivatives
Atoms
Chemical analysis

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

これを引用

A new trisaccharide sugar chain linked to a serine residue in bovine blood coagulation factors VII and IX. / Hase, Sumihiro; Kawabata, Shun-Ichiro; Nishimura, Hitoshi; Takeya, Hiroyuki; Sueyoshi, Tatsuya; Miyata, Toshiyuki; Iwanaga, Sadaaki; Takao, Toshifumi; Shimonishi, Yasutsugu; Ikenaka, Tokuji.

:: Journal of Biochemistry, 巻 104, 番号 6, 01.01.1988, p. 867-868.

研究成果: ジャーナルへの寄稿記事

Hase, S, Kawabata, S-I, Nishimura, H, Takeya, H, Sueyoshi, T, Miyata, T, Iwanaga, S, Takao, T, Shimonishi, Y & Ikenaka, T 1988, 'A new trisaccharide sugar chain linked to a serine residue in bovine blood coagulation factors VII and IX', Journal of Biochemistry, 巻. 104, 番号 6, pp. 867-868. https://doi.org/10.1093/oxfordjournals.jbchem.a122571
Hase, Sumihiro ; Kawabata, Shun-Ichiro ; Nishimura, Hitoshi ; Takeya, Hiroyuki ; Sueyoshi, Tatsuya ; Miyata, Toshiyuki ; Iwanaga, Sadaaki ; Takao, Toshifumi ; Shimonishi, Yasutsugu ; Ikenaka, Tokuji. / A new trisaccharide sugar chain linked to a serine residue in bovine blood coagulation factors VII and IX. :: Journal of Biochemistry. 1988 ; 巻 104, 番号 6. pp. 867-868.
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