A new type of O2--generating tool for oxidative stress studies by remodeling neutrophil NADPH oxidase

Minoru Tamura, Teruaki Nagasawa, Tomoyuki Tange, Kei Miyano, Shin Ichi Kobayashi, Toshiki Nakai, Satoshi Miura

研究成果: ジャーナルへの寄稿記事

3 引用 (Scopus)

抄録

The effects of reactive oxygen species on cells have attracted much attention in relation to redox regulation and oxidative stress-related diseases. Superoxide (O2-) is the reactive oxygen species primarily formed in biological systems. However, no convenient O2 --generating device has been available for use in cell or tissue culture. The neutrophil NADPH oxidase, a professional enzyme for killing bacteria, has a high ability to produce O2-. However, the cell-free activation process requires several protein factors and an anionic amphiphile, and moreover, the activation is transient. To utilize the enzyme as an O2- generator, we improved the cell-free activation method by remodeling regulatory components, optimizing lipid composition, and modifying the mixing conditions. We established a new method to produce an active enzyme that is stable, efficient, and preservable. As an application, we examined the effect of the device on cultured HEK293 cells and observed that it caused cell death. This system has several advantages over the xanthine oxidase system often used. The new device will be useful for studies of oxidative stress and related diseases.

元の言語英語
ページ(範囲)421-429
ページ数9
ジャーナルJournal of Biotechnology
120
発行部数4
DOI
出版物ステータス出版済み - 12 6 2005

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Oxidative stress
NADPH Oxidase
Oxidative Stress
Neutrophils
Enzymes
Chemical activation
Reactive Oxygen Species
Equipment and Supplies
Tissue culture
Amphiphiles
Oxygen
Xanthine Oxidase
Cell death
Biological systems
Cell culture
Superoxides
Lipids
HEK293 Cells
Bacteria
Oxidation-Reduction

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

これを引用

Tamura, M., Nagasawa, T., Tange, T., Miyano, K., Kobayashi, S. I., Nakai, T., & Miura, S. (2005). A new type of O2--generating tool for oxidative stress studies by remodeling neutrophil NADPH oxidase. Journal of Biotechnology, 120(4), 421-429. https://doi.org/10.1016/j.jbiotec.2005.06.035

A new type of O2--generating tool for oxidative stress studies by remodeling neutrophil NADPH oxidase. / Tamura, Minoru; Nagasawa, Teruaki; Tange, Tomoyuki; Miyano, Kei; Kobayashi, Shin Ichi; Nakai, Toshiki; Miura, Satoshi.

:: Journal of Biotechnology, 巻 120, 番号 4, 06.12.2005, p. 421-429.

研究成果: ジャーナルへの寄稿記事

Tamura, M, Nagasawa, T, Tange, T, Miyano, K, Kobayashi, SI, Nakai, T & Miura, S 2005, 'A new type of O2--generating tool for oxidative stress studies by remodeling neutrophil NADPH oxidase', Journal of Biotechnology, 巻. 120, 番号 4, pp. 421-429. https://doi.org/10.1016/j.jbiotec.2005.06.035
Tamura, Minoru ; Nagasawa, Teruaki ; Tange, Tomoyuki ; Miyano, Kei ; Kobayashi, Shin Ichi ; Nakai, Toshiki ; Miura, Satoshi. / A new type of O2--generating tool for oxidative stress studies by remodeling neutrophil NADPH oxidase. :: Journal of Biotechnology. 2005 ; 巻 120, 番号 4. pp. 421-429.
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